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An extended chemical analysis of gallstone

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Abstract

Chemical composition of gall stones is essential for aetiopathogensis of gallstone disease. We have reported quantitative chemical analysis of total cholesterol bilirubin, calcium, iron and inorganic phosphate in 120 gallstones from haryana. To extend this chemical analysis of gall stones by studying more cases and by analyzing more chemical constituents. A quantitative chemical analysis of total cholesterol, total bilirubin, fatty acids, triglycerides, phospholipids, bile acids, soluble proteins, sodium potassium, magnesium, copper, oxalate and chlorides of biliary calculi (52 cholesterol, 76 mixed and 72 pigment) retrieved from surgical operation of 200 patients from Haryana state was carried out. Total cholesterol as the major component and total bilirubin, phospholipids, triglycerides, bile acids, fatty acids (esterified), soluble protein, calcium, magnesium, iron, copper, sodium, potassium, inorganic phosphate, oxalate and chloride as minor components were found in all types of calculi. The cholesterol stones had higher content of total cholesterol, phospholipids, fatty acids (esterified), inorganic phosphate and copper compared to mixed and pigment stones. The mixed stones had higher content of iron and triglycerides than to cholesterol and pigment stones. The pigment stones were richer in total bilirubin, bile acids, calcium, oxalate, magnesium, sodium, potassium, chloride and soluble protein compared to cholesterol and mixed stones. Although total cholesterol was a major component of cholesterol, mixed and pigment gall stone in Haryana, the content of most of the other lipids, cations and anions was different in different gall stones indicating their different mechanism of formation.

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References

  1. Raha PK, Sengupta KP, Aikat BK. X-Ray Diffraction analysis of gallstones. Ind J Med Res 1966; 54: 729–34

    Google Scholar 

  2. Udupa KN, Chansouria JPN, Gode JD, Gupta S. Studies on etiology of gallstone. Ind J Surg 1968; 68: 120–28

    Google Scholar 

  3. Tyagi SP, Tyagi N, Maheshwari V, Ashraf SM, Sahoo P. Morphological changes in diseased gall bladder: A study of 415 cholecystectomies at Aligarh. J Ind Med Assoc 1992; 90: 178–81

    CAS  Google Scholar 

  4. Bansal SK, Gupta SK, Bansal A, Rajput VS, Joshi LD. Chemical composition of Biliary Calculi from Kanpur region. Ind J Clin Biochem 1992; 7: 27–9

    Article  Google Scholar 

  5. Bhansali SK. Choledochostomy. Ind J Surg, 1979; 41: 485–91

    Google Scholar 

  6. Singh A, Bagga SPS, Jindal VP, Singh K, Rao SS. Gall bladder disease: An analytic report of 250 cases. J Ind Med Assoc, 1989; 87: 253–6

    CAS  Google Scholar 

  7. Verma GR, Pandey AK, Bose SM, Prasad R. Study of serum calcium and trace elements in chronic cholelithiasis. ANZ J Surg 2002; 72: 596–9

    Article  PubMed  Google Scholar 

  8. Pundir CS, Chaudhary R, Rani K, Chandran P, Kumari M, Garg P. Chemical analysis of biliary calculi in Haryana. Ind J Surg 2001; 63: 370–73

    Google Scholar 

  9. Allain CC, Poon LS, Chan CS, Richmond W, Fu PC. Enzymatic determination of total serum cholesterol. Clin Chem 1974; 20: 470–75

    PubMed  CAS  Google Scholar 

  10. Gambino SR. In “Standard methods of clinical chemistry”. Meiter S. Ed., Academic Press. 1965; 5: 55

  11. Buccolo G, David H. Quantitative determination of serum triglycerides by the use of enzymes. Clin Chem 1973; 20: 470–75

    Google Scholar 

  12. Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J Biol Chem 1951; 193: 265–75

    PubMed  CAS  Google Scholar 

  13. Satyapal, Pundir CS. Purification and properties of an oxalate oxidase from leaves of grain sorghum hybrid CSH-5. Biochem Biophys Acta 1993; 1161: 1–5.

    PubMed  CAS  Google Scholar 

  14. Young SD, Pestaner LC, Gibberman V. Effect of drugs on clinical laboratory tests. Clin Chem 1975; 21: 5.

    Google Scholar 

  15. Fiske CH, Subba Row Y. The colorimetric determination of phosphorous. J Biol Chem 1925; 66: 375–400

    CAS  Google Scholar 

  16. Neill DW, Neely RA. Titan yellow method for magnesium. J Clin Pathol, 1956; 9: 162 cited in Hawk's Physiological Chemistry. Ed. B.L. Oster, 4th Edition—The Blackiston Division, McGraw Hill Book Co. 1965, p. 1139

    Article  PubMed  CAS  Google Scholar 

  17. Schoenfeld RG. Colourimetric method for chloride determination. Clin Chem 1964; 10: 533.

    PubMed  CAS  Google Scholar 

  18. Stern, Shapiro. Practical Clinical Biochemistry by Harrold Varley. Fourth edition, Heinemann Professional Publishing Ltd., London 1953.

    Google Scholar 

  19. Carey JB. The serum trihydroxy-dihydroxy bile acid ratio in liver and biliary tract disease. J Clin Invest 1958; 17: 1494–1502.

    Article  Google Scholar 

  20. Goswami M. An analysis of 160 cholecystectomies at Guwahati. Ind J Surg 1999; 61: 252–5

    Google Scholar 

  21. Nakayama F. Quantitative microanalysis of gallstones. J Lab Clin Med 1968; 72: 602–11

    PubMed  CAS  Google Scholar 

  22. Ti JK, Yuen R. Chemical composition of biliary calculi in relation to pattern of biliary disease in Singapore. Br J Surg 1985; 72: 556–8.

    Article  PubMed  CAS  Google Scholar 

  23. Rains AJH. Gallstones. Heinemann Monography. Ist ed., 1964

  24. Horn G. Observations on the aetiology of cholelithiasis. Bri Med J 1965; 2: 732

    Google Scholar 

  25. Moore EW. The role of calcium in the pathogenesis of gallstones. Hepatology 1984; 4: 228–43.

    Article  Google Scholar 

  26. Cuevas A, Miquel JF, Reyes MS. Diet as a risk factor for cholesterol gall stone disease. J Am Coll Nutr 2004; 23: 187–96.

    PubMed  Google Scholar 

  27. Kumar D, Garg PK, Tandon RK. Clinical and biochemical comparative study of different types of common bile duct stones. Ind J Gastroenterol 2001; 20: 187–90

    CAS  Google Scholar 

  28. Trotman BW, Ostrow JD, Soloway RH. Pigment versus cholesterol cholelithiasis: Comparison of stone and bile composition. Am J Dig Dis 1974; 19: 585–90

    Article  PubMed  CAS  Google Scholar 

  29. Lamont. Mucin glycoprotein content of human pigment stone. Hepatology 1983; 3: 372–82

    Google Scholar 

  30. Binette JP, Binette MB. The proteins and the formation of gallstones. Clin Chim Acta 2000; 296: 59–69

    Article  PubMed  CAS  Google Scholar 

  31. Maki T. Pathogenesis of calcium bilirubinate gallstones: role of E. Coli beta-glucoronidase and coagulation by inorganic ions, polyelectrolytes and agitation. Annal Surg 1966; 164: 90–100

    Article  CAS  Google Scholar 

  32. Amin AM, Ananthakrishnan N, Nambinarayanan TK. Composition of gallstones and Sequential events in biliary lithogenesis—is it different in South India compared to north? J Assoc Physicians India 2000; 48: 885–90.

    PubMed  CAS  Google Scholar 

  33. Been JM, Bills PM, Lewis D. Electron Probe microanalysis in the study of gallstones. Gut 1977; 18: 836–42.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Biochemistry Research Laboratory, Department of Bio-Sciences, M.D. University, 124 001, Rohtak, Haryana, India

    P. Chandran, N. K. Kuchhal & C. S. Pundir

  2. Department of Surgery, Pt BDS P.G.I.M.S., 124001, Rohtak, India

    P. Garg

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  1. P. Chandran
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  2. N. K. Kuchhal
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  3. P. Garg
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  4. C. S. Pundir
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Correspondence to C. S. Pundir.

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Chandran, P., Kuchhal, N.K., Garg, P. et al. An extended chemical analysis of gallstone. Indian J Clin Biochem 22, 145–150 (2007). https://doi.org/10.1007/BF02913334

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