Biologia Plantarum

, Volume 37, Issue 2, pp 329–334 | Cite as

Overcoming prefertilization barriers in the crossDiplotaxis siettiana ×Brassica juncea using irradiated mentor pollen

  • B. K. Sarmah
  • N. Sarla
Original Papers


Hybridization between the nearly extinct speciesDiplotaxis siettiana andBrassica juncea is prevented because of strong prefertilization barriers. Use of mentor pollen ofD. siettiana irradiated with 1000 Gy gamma radiation before the incompatible pollination led to fertilization. 5 d after pollination 17% ovules showed entry of pollen tubes, 10 d after pollination 27% ovules showed small globular embryos which grew no further. No embryos were found in control pollinations. Thus , use of irradiated mentor pollen brings about fertilization in this difficult cross and hybrids can be obtained if embryos are rescued.

Key words

gamma radiation incompatibility pollen germination 



days after pollination


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Copyright information

© Institute of Experimental Botany, ASCR 1995

Authors and Affiliations

  • B. K. Sarmah
    • 1
  • N. Sarla
    • 1
  1. 1.Division of GeneticsIndian Agricultural Research InstituteNew DelhiIndia

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