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Overcoming prefertilization barriers in the crossDiplotaxis siettiana ×Brassica juncea using irradiated mentor pollen

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Biologia Plantarum

Abstract

Hybridization between the nearly extinct speciesDiplotaxis siettiana andBrassica juncea is prevented because of strong prefertilization barriers. Use of mentor pollen ofD. siettiana irradiated with 1000 Gy gamma radiation before the incompatible pollination led to fertilization. 5 d after pollination 17% ovules showed entry of pollen tubes, 10 d after pollination 27% ovules showed small globular embryos which grew no further. No embryos were found in control pollinations. Thus , use of irradiated mentor pollen brings about fertilization in this difficult cross and hybrids can be obtained if embryos are rescued.

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Abbreviations

dap:

days after pollination

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Sarmah, B.K., Sarla, N. Overcoming prefertilization barriers in the crossDiplotaxis siettiana ×Brassica juncea using irradiated mentor pollen. Biol Plant 37, 329–334 (1995). https://doi.org/10.1007/BF02913236

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  • DOI: https://doi.org/10.1007/BF02913236

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