Abstract
Hybridization between the nearly extinct speciesDiplotaxis siettiana andBrassica juncea is prevented because of strong prefertilization barriers. Use of mentor pollen ofD. siettiana irradiated with 1000 Gy gamma radiation before the incompatible pollination led to fertilization. 5 d after pollination 17% ovules showed entry of pollen tubes, 10 d after pollination 27% ovules showed small globular embryos which grew no further. No embryos were found in control pollinations. Thus , use of irradiated mentor pollen brings about fertilization in this difficult cross and hybrids can be obtained if embryos are rescued.
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Abbreviations
- dap:
-
days after pollination
References
Batra, V., Prakash, S., Shivanna, K.R.: Intergeneric hybridization betweenDiplotaxis siifolia, a wild species and crop Brassicas.—Theor. appl. Genet.80: 537–541, 1990.
Delourme, R., Eber, F., Chevre, A.M.: Intergeneric hybridization ofDiplotaxis erucides withBrassica napus. I. Cytogenetic analysis of F1 and BC1 progeny.—Euphytica41: 123–128, 1989.
Gomez-Campo, C.: A germplasm collection of crucifers.—Ministerio de Agricultura Pesca y Alimentacion. Instituto Nacional de Investigaciones, Agrarias. Catalogos I.N.I.A. No. 22., Madrid 1990.
Hinata, K., Konno, N.: Studies on a male sterile strain having theBrassica campestris nucleus and theDiplotaxis muralis cytoplasm.—Jap. J. Breed.29: 305–311, 1979.
Knox, R.B., Gaget, M., Dumas, S.: Mentor pollen techniques.—Int. Rev. Cytol.107: 315–332, 1987.
Nandakumar, P.B.A., Shivanna, K.R.: Intergeneric hybridization betweenDiplotaxis siettiana and crop brassicas for the production of alloplasmic lines.—Theor. appl. Genet.85: 770–776, 1993.
Ringdhal, E.A., McVetty, P.B.E., Sernyx, J.L.: Interspecific hybridization ofDiplotaxis spp. withBrassica napus: A source of new cms system.—Can. J. Plant Sci.67: 239–243, 1987.
Takahata, Y., Hinata, K.: Studies on cytodemes in subtribe Brassicae (Cruciferae).—Tohoku J. agr. Res.33: 111–124, 1983.
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Sarmah, B.K., Sarla, N. Overcoming prefertilization barriers in the crossDiplotaxis siettiana ×Brassica juncea using irradiated mentor pollen. Biol Plant 37, 329–334 (1995). https://doi.org/10.1007/BF02913236
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DOI: https://doi.org/10.1007/BF02913236