Effect ofOcimum sanctum (Tulsi) and vitamin E on biochemical parameters and retinopathy in streptozotocin induced diabetic rats
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This study was carried out to see the effect of the aqueous extract ofOcitum sanctum Linn (Tulsi) with Vitamin E on biochemical parameters and retinopathy in the streptozotocin-induced diabetic albino male rats. Adult albino male rats weighing 150–200 gm were made diabetic by intraperitoneal injection of streptozotocin in the dose 60 mg/kg in citrate buffer (pH 6.3). The diabetic animals were left for one month to develop retinopathy. Biochemical parameters like plasma glucose, oral glucose tolerance and glycosylated hemoglobin HbA1c, were measured along with lipid profile, and enzymes like glutathione peroxidase (GPX), lipid peroxidase (LPO), superoxide dismutase (SOD), catalase (CAT) and glutathione-S-transferase (GST) in normal, untreated diabetic rats and diabetic rats treated withOcimum sanctum L extracts and vitamin E. Fluorescein angiography test was done for assessing retinopathy. Results on biochemical parameters were analyzed statistically by using ANOVA followed by Dunnet's ‘t’-test. A p-value of <0.05 was considered as significant. Evaluation of biochemical profile in treated groups showed statistically significant reduction in plasma levels of glucose, HbA1c, lipid profile and LPO, and elevation of GPX, SOD, CAT and GST. Treatment of the diabetic animals withOcimum sanctum and Vitamin E, alone and in combination for 16 weeks showed reversal of most of the parameters studied including plasma glucose levels. Angiography showed improvement in retinal changes following combined antidiabetic treatment.
Key WordsOcimum sanctum Vitamin E Diabetes mellitus Diabetic retinopathy Lipid peroxidation Antioxidants
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- 2.Klein, R. and Klein, B.E.K. (1995) Vision disorders in diabetes. In Diabetes in America. 2nd ed. National Diabetes Data Group, Ed. Bethesda, M D, NIH (Pub 1). 95 (1468), 293–338.Google Scholar
- 4.Riordan, E. P, Eye. (2003) In current Medical Diagnosis and Treatment. 42nd ed. Tierney L. M., Mcphee, S. J., Papadakis, M.A., Eds. (New York), Lange Medical Books Mc Graw-Hill, 46, 177–1778.Google Scholar
- 9.Sivaram, P. (1994) In New Developments in cardiology and cardiac surgery. Eds. Tehran, N., Kumar, A. Escort Heart Institute and Research Center, (New Delhi), 1.Google Scholar
- 10.Jain, S.K. (1996) The effect of modest vitamin E supplementation on lipid peroxidation products and other cardiovascular risk factors in diabetic patients. Lipids, suppl S. 86, 90–94.Google Scholar
- 11.Eshrat, Halim M.A. Hussain, Kaiser Jamil and Mala Rao. (2001) Hypoglycemic, Hypolipidemic and antioxidant properties of Tulsi (Ocimum sanctum Linn) on streptozotocin induced diabetes in rats. Ind J Clin Biochem, 2, 190–194.Google Scholar
- 13.Eshrat, H. M. (2003) Lowering of blood sugar by water extract ofAzadirachta indica andAbroma augusta in diabetes rats. Ind.J. Exp. Biol, 41, 636–640.Google Scholar
- 15.Aeibi, H., Catalase In., H.U., Berg, Meyer (ed). (1974) Methods in Enzymatic Analysis. Academic Press, New York, 673–684.Google Scholar
- 24.Wilkinson, C.P. (1986) The clinical examination. Limitation and over utilization of Angiographic services. Ophthalmology, 93, 410–404.Google Scholar
- 25.Garg, M.C., Ojha, S. and Bansal, D.D. (1996) Antioxidant status of streptozotocin diabetic rats. Ind. J. Exp. Biol, 34, 264–266.Google Scholar
- 27.The PKC-DRS Study Group (2005) Initial Results of the Protein Kinase C b Inhibitor Diabetic Retinopathy Study (PKC-DRS). Multicenter Randomized Clinical Trial Diabetes, 54, 2188–2197Google Scholar
- 28.Cusick, M., Chew, E.Y., Clemons, T.E., Klein, R., Klein, B.E.K. and Hubbard, L.D. (2002) Effect of antioxidant and Zinc supplements on development of diabetic retinopathy in the Age Related Eye Disease Study (AREDS). Invest Ophthalmol Vis Sci 43: E 1909.Google Scholar