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Biochemical activity of selenium and glutathione on country made liquor (CML) induced hepatic damage in rats

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Abstract

The serum and hepatic enzymes of rats were studied after exposed to country made liquor (CML) along with two chelating agents (glutathione and Selenium). There was a significant increase in several serum enzyme levels (viz., aspartate transaminase, alanine transaminase, alkaline phosphatase, sorbitol dehydrogenase, glutamate dehydrogenase, bilirubin) and decrease in various hepatic enzymes (Succinic dehydrogenase, Glucose 6-phosphatase, 5'Nucleotiease, Acid phosphatase, Acid ribonuclease, Cytochrome P-450) due to repeated administration of CML (2ml/100g of body weight). Results of this study revealed that the GSH and Se could give a significant protective action in serum and hepatic enzymes of CML exposed rats.

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References

  1. Nevins, CL, Malaty H, Velez ME, Anand BS. Interaction of alcohol and hepatitis C virus infection on severity of liver disease. Dig Dis and Sci 1999: 1236–42.

  2. Rainke LA, Mc Cay PB. Spin trapping studies of alcohol initiated radicals in rat lever; influence of dietary fat. In American society for Nutrition Sciences annual meeting Journal of Nutrition, 1997: 127.

  3. Bellentani S, Saccocio G, Masutti F, Giacca M, Miglioli L, Monzoni A, Tiribelli C. Risk factors for alcoholic liver disease. Addict Biol 2000; 5(3): 261–8.

    Article  Google Scholar 

  4. Fickert P, Zatloukal K. Pathogenesis of alcoholic liver disease. In: Zernig G, Saria A, Kurz M, Malley SSO, editors. Handbook of alcoholism, CRS Press, 2000; 317–23.

  5. Yang Z, Dovis A, Harley Z, Stone C, Bosten W. Catalytic efficiency of human alcohol dehydrogenase for retinal oxidation and retinol reductions. Alc Clin Exp Res 1994; 18(3): 587–91.

    Article  CAS  Google Scholar 

  6. National Institute of Health Guide for the Care and Use of laboratory Animals. DHEW Publication (NIH), revised, Office of Science and Health Reports, DRR/NIH, Bethesda, USA, 1985.

    Google Scholar 

  7. Reitman S, Frankel S. A colorimetric method for the determination of serum glutamic oxaloactic acid and glutamic pyruvic transaminases. Am J Clin Pathol 1957; 28: 56–63.

    PubMed  CAS  Google Scholar 

  8. Kind PRN, King EJJ. Estimation of plasma phosphatase by determination of hydrolyzed phenol with aminopyrines. J Clin Pathol 1954; 7: 332.

    Article  CAS  Google Scholar 

  9. Gerlach U, Hiby W. Sorbitol dehydrogenase. In: Bergmeyer H. U, editors. Methods of Enzymatic analysis. Vol. II. Verlag Chemie Weinheim, Academic Press, New York. 1974; 569–73.

    Google Scholar 

  10. Malloy HT, Evelyn KA. The determination of bilirubin with the photometric colorimeter. J Biol Chem 1937; 119: 481–90.

    CAS  Google Scholar 

  11. Green DE, Mii S, Kohout PM. Studies on the terminal electron transport system. I. Succinic dehydrogenase. J Biol Chem 1955; 217: 551–67.

    PubMed  CAS  Google Scholar 

  12. Baginski ES, Foa PP, Zak B. Glucose-6-phosphatase. In: Bergmeyer H. U, editor. Methods of Enzymatic analysis. Vol. II. Verlag Chemie Weinheim, Academic Press, New York 1974; 876–80.

    Google Scholar 

  13. Aronson NN Jr, Touster O. Isolation of rat liver plasma membrane fragment in isotonic sucrose. In: Fleischer S, Packer L, editors. Methods in Enzymol, Vol. 31. Academic Press, New York 1974; 90–2.

    Google Scholar 

  14. De Duve C, Pressman BC, Gianetto R, Wattiaux R, Appelmans F. Tissue fractionation studies. Intercellular distribution pattern of enzymes in rat liver tissue. Biochem J 1955; 60: 604–17.

    Google Scholar 

  15. Schneider WC, Hogeboom GH. Intracellular distribution of enzymes. Further studies on the distribution of cytochrome C in rat liver homogenates. J Biol Chem 1950; 183: 123–8.

    CAS  Google Scholar 

  16. Omura T, Sato R. The carbon monoxide binding pigment of liver microsomes. J Biol Chem 1964; 239: 2370–8.

    PubMed  CAS  Google Scholar 

  17. Kumar G, Sharmila Banu G, Kannan V. Rajasekara Pandian M. Antihepatotoxic effect of â-carotene on paracetamol induced hepatic damage in rats. Ind J Exp Biol 2005; 43: 351–5.

    CAS  Google Scholar 

  18. Chhya Gadgoli, Mishra SH. Antihepatotoxic activity of pmethoxy benzoic acid fromCapparis spinosa. J Ethnopharmacol 1999; 66: 187–92.

    Article  PubMed  CAS  Google Scholar 

  19. Tanaka K, Lizuka Y. Suppression of enzyme release from isolated rat liver lysosomes by non-steroidal antiinflammatory drugs. Biochem Pharmacol 1968; 17: 3023–32.

    Google Scholar 

  20. Mandal PK, Bishayee A, Chatterjee M. Stimulation of tissue repair byMikania cordata root extract in carbon tetrachloride induced liver injury in mice. Phytother Res 1993; 7: 103–5.

    Article  Google Scholar 

  21. Yeragi SG, Rana AM, Koli VA. Effect of pesticides on protein metabolism of MudskipperBoleophthalmus Dussumieri. J Ecotoxicol Environ Monit 2003; 13: 211–4.

    CAS  Google Scholar 

  22. Bradbury SP, Mc Kim JM, Coats JR. Physiological responses of rainbow trout, Salmogairdneri to acute fenvalerate intoxication. Pest Biochem Physiol 1987; 27: 258.

    Article  Google Scholar 

  23. Lieber CS. Alcohol and liver in medical disorders of alcoholism. In: Pathogenesis and Treatment, Smith LH, editor. W.B. Sounders Co., Philadelphia. 1982; 259–64.

    Google Scholar 

  24. Renzoni R. Mercury and selenium in the Mediterranean Sea. 5th Joint oceanographic assembly abstracts. Vol. I. Department of Fisheries and Oceans, Scientific Information and Publication Branch, Ottawa, 1982; 53.

    Google Scholar 

  25. Chandy JP, Patel B. Do selenium and Glutathione (GSH) detoxify mercury in marine invertebrates? Effects on lysosomal response in the tropical blood clamAnadars granosa. Dis Aqua Org 1985; 1: 39–47.

    Article  CAS  Google Scholar 

  26. Kumar G, Sharmila Banu G, Kannan V. Effects of Arrack on liver protein ofMus musculus. J Ecobiol 2006; 18: 321–4.

    Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, Selvamm Arts & Science College, Namakkal

    G. Kumar

  2. Centre for Biotechnology, Muthay ammal College of Arts and Sciences, Kakkaveri, Rasipuram, 637408, Namakkal, Tamilnadu, India

    G. Sharmila Banu & M. Rajasekara Pandian

Authors
  1. G. Kumar
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  2. G. Sharmila Banu
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  3. M. Rajasekara Pandian
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Correspondence to M. Rajasekara Pandian.

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Kumar, G., Banu, G.S. & Pandian, M.R. Biochemical activity of selenium and glutathione on country made liquor (CML) induced hepatic damage in rats. Indian J Clin Biochem 22, 105–108 (2007). https://doi.org/10.1007/BF02912891

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