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Treatment of streptozotocin induced diabetes in male rats by immunoisolated transplantation of islet cells

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Abstract

Insulin injection is the main way to combat against insulin-dependent diabetes mellitus effects. Today in some laboratories in the world, the investigators are trying to find some treatments for this disease with insulin-secreting pancreatic islet cells transplantation. Donor tissue in each step of work was prepared from 36 adult male wistar Rats weighted 250–300 grams (75–90 days). Transplantation was done in rats after 2–4 weeks induction of diabetes with 60mg/kg of streptozotocin injection by intravenous method. Encapsulation of pancreatic islet cells allows for transplantation in the absence of immunosuppression. This technique that is called “immunoisolation” is based on the principle that transplanted tissue is protected for the host immune system by an artificial or natural membrane. In this study, the levels of insulin, C-peptide and glucose in diabetic rats have been reached to normal range as compared to un-diabetic rats in 20 days after transplantation of islet cells, so that testis is immunoisolated place for islet cells transplantation. Inside the testis subcutaneously and intrapretoneally implantation of pure islet cells graft, that is a natural immunoisolation method, rapidly and permanently normalized the diabetic state of streptozocin-administered animals.

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Authors and Affiliations

  1. Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

    Sh. Jamshidi

  2. Biochemistry Department of Alzahra University, Tehran, Iran

    B. Lame Rad

  3. Biometric Department, S.P.I.I. Institute, Karaj, Iran

    M. Mofidian

  4. Department of Pilot Biotechnology, Pasteur Institute of Iran, 13164, Tehran, Iran

    A. Akbarzadeh, D. Noruzian, A. Farhangi, M. R. Mehrabi & A. Allahverdi

Authors
  1. A. Akbarzadeh
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  2. D. Noruzian
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  3. Sh. Jamshidi
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  4. A. Farhangi
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  5. M. R. Mehrabi
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  6. B. Lame Rad
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  7. M. Mofidian
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  8. A. Allahverdi
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Correspondence to A. Akbarzadeh.

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Akbarzadeh, A., Noruzian, D., Jamshidi, S. et al. Treatment of streptozotocin induced diabetes in male rats by immunoisolated transplantation of islet cells. Indian J Clin Biochem 22, 71–76 (2007). https://doi.org/10.1007/BF02912885

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