Abstract
The present study was undertaken to investigate the effect of oxidized low density lipoprotein (oxLDL) on the expression of macrophage matrix metalloproteinase-12 (MMP-12), and the possible mechanisms. Activation of extracellular signal-regulated kinase 1/2 (ERK1/2) was detected by Western blot Analysis. Enzymatic activity of MMP-12 was determined by β-casein zymography. RT-PCR analysis was used to measure the mRNA expression level of MMP-12. OxLDL-stimulated macrophages produced increased casein-degrading activities and oxLDL also significantly increased the mRNA level of MMP-12 in a dose-dependent manner. OxLDL stimulated the phosphorylation of ERK1/2 in macrophages. The use of the specific inhibitor indicated that the ERK1/2 signaling pathway was required for the induction of MMP-12. These data demonstrated that oxLDL induced MMP-12 expression in macrophages through an ERK1/2-dependent pathway.
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Foundation item: Supported by the Natural Science Foundation of Hubei Province (99J138)
Biography: He Chun-yan (1970-), female, Ph. D candidate, research direction: biochemistry diagnosis of atherosclerosis.
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Chun-yan, H., Xin, Z., Xiao-ming, L. et al. Activation of proteinkinase ERK mediates induction of macrophage MMP-12 by OxLDL. Wuhan Univ. J. Nat. Sci. 9, 120–124 (2004). https://doi.org/10.1007/BF02912732
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DOI: https://doi.org/10.1007/BF02912732