Immunohistochemical analysis of TNF-α and HSP-60 in women with tubal factor infertility associated withChlamydia trachomatis

  • Zhao Haizhen
  • Li Hongfa


To explore the roles of tumor necrosis factor-α (TNF-α) and heat shock protein 60 (HSP-60) in women with tubal factor infertility (TFI) associated withChlamydia trachomatis, and to determine the mechanisms of fallopian adhesions inChlamydia trachomatis (CT) infections, the expressions of TNF-α and HSP-60 were quantitatively determined in 60 cases of TFI and 30 controls by immunohistochemical technique. The patients with TFI were further divided into group A and group B according to the CT-DNA of cervical specimens of PCR. The quantitative analysis was conducted by employing computerized image analysis system. It is found that the expressions of TNF-α and HSP-60 were much higher in TFI patients than those of controls. Among CT-HSP responders, a stronger expression was correlated with more severe salpingeal pathology. It is concluded that TNF-α and HSP-60 play very important roles in fallopian tube adhesion and occlusion in TFI due to CT infection.

Key words

TFI HSP-60 TNF-α Chlamydia trachomatis immunohistochemistry image analysis 


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Copyright information

© Springer 2004

Authors and Affiliations

  • Zhao Haizhen
    • 1
  • Li Hongfa
    • 1
  1. 1.Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina

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