Summary
The National Institute of Diabetes, Digestive and Kidney Diseases (NIDDK) provides the hFSH-I-3 preparation, to be employed as a tracer in the radioimmunoassay (RIA) of human follicle-stimulating hormone (hFSH). The contaminating LH contained in that preparation led us to study whether the iodination of such a material could render it a suitable tracer for RIA of both LH and FSH. hFSH-I-3 was labelled with125I by the chloramine-T method and was further purified on Sephadex G-75 column. The LH-RIA was performed using this preparation and anti-LH at a final dilution of 1:37,500, with a sensitivity of 3 mIU LH/ml (2nd international reference pattern). The method was validated by comparing the LH values obtained in different serum samples with those obtained using the standard RIA (125I-LH/anti-LH); the correlation coefficient (r) was equal to 0.9988. No LH overestimation due to the putative cross-reaction with FSH was found. This was demonstrated by testing serum samples containing high (>100 mIU/ml) and low (<10 mIU/ml) concentrations of FSH before and after the treatment with anti-LH. Under these conditions, serum samples from postmenopausal women, pregnant women, normal men and women in basal conditions and after the LH-RH administration, and from a patient with Klinefelter’s syndrome, were evaluated. In conclusion, NIDDK125I-hFSH-I-3 can be used as a tracer for the radioimmunological quantitation of both hLH and hFSH, which results not only inexpensive, but also allows to reduce the amount of the stored radioactive materials.
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References
Hunter W. M., Greenwood F. C.: Preparation of iodine 131-labeled human growth hormone of high specific activity — Nature (Lond.)194, 495, 1962.
Midgley A. R. Jr.: Radioimmunoassay: a method for human chorionic gonadotropin and human luteinizing hormone — Endocrinology79, 10, 1966.
Midgley A. R. Jr.: Radioimmunoassay for human follicle-stimulating hormone — J. clin. Endocrinol.27, 295, 1967.
Rodbard D.: Statistical quality control and routine data processing for radioimmunoassay and immunoradiometric assay — Clin. Chem.20, 1255, 1976.
Scaglia H. E., Medina M., Pinto-Ferreira A. L., Vázquez G., Gual C., Pérez-Palacios G.: Pituitary LH and FSH secretion and responsiveness in women of old age — Acta endocrinol. (Kbh.)81, 673, 1976.
Scaglia H. E., Ramirez J. R., Mendoza F., Pérez-Palacios G.: Gonadotropin dynamics in Klinefelter’s syndrome — Reproduction2, 7, 1975.
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Mariani, V., Aquilano, D.R. & Scaglia, H.E. Radioimmunoassays of human luteinizing and follicle-stimulating hormones using the same radioactive tracer. La Ricerca Clin. Lab. 17, 163–169 (1987). https://doi.org/10.1007/BF02909411
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DOI: https://doi.org/10.1007/BF02909411