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Modulation of β-adrenergic receptor by hydrocortisone in human polymorphonuclear leukocytes

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Ricerca in clinica e in laboratorio

Summary

We have examined the influence of isoproterenol and hydrocortisone on the cyclic AMP content and lysosomal enzyme release from highly purified human polymorphonuclear leukocytes. It was found that isoproterenol, although a potent stimulus for cAMP accumulation in human lymphocytes, had only marginal effects on both cAMP content of PMA and enzyme release. Since corticosteroids have been reported to increase the response to β-agonists in various tissues, it was considered that some of the anti-inflammatory potential of corticosteroids might be related to enhancing the response of PMN to β-agonists. The influence of hydrocortisone on the response to isoproterenol was accordingly examined. It was found that hydrocortisone did in fact augment the isoproterenol-induced increase in cAMP content. In contrast to the potentiation of cAMP accumulation caused by hydrocortisone and isoproterenol together, the two agents produced only an additional inhibition of enzyme release. Since the inhibition of lysosomal enzyme release from human PMN did not closely reflect the changes observed in the cAMP content, a possible compartmentalization of intracellular cAMP is suggested.

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Authors and Affiliations

  1. Istituto di Patologia Medica II Facoltà di Medicina e Chirurgia, Università degli Studi di Napoli, Via S. Pansini 5, 80131, Napoli, Italia

    Gianni Marone & Mario Condorelli

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  1. Gianni Marone
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  2. Mario Condorelli
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This work was supported in part by grants no. 79.02392.65 and 80.00501.04 from theConsiglio Nazionale delle Ricerche (CNR), Roma, Italy.

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Marone, G., Condorelli, M. Modulation of β-adrenergic receptor by hydrocortisone in human polymorphonuclear leukocytes. La Ricerca Clin. Lab. 12, 469–477 (1982). https://doi.org/10.1007/BF02909398

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