Summary
Tuberculosis is still one of the major health problems in almost all over the world. Thus, new directions in basic and applied research on tuberculosis are under investigation. In this review we have provided recent data obtained in our laboratories on three main aspects of the immunology of tuberculosis, namely:i. the role of B lymphocytes in the processing and presentation ofMycobacterium tuberculosis antigens to T cells;ii. the activation and characterization of mycobacterial-specific T cell clones;iii. the T cell regulation of the immune response toM. tuberculosis. The analysis of the antigenic determinants ofM. tuberculosis relevant in the antimycobacterial immunity is the major goal of the WHO programme on the immunology of tuberculosis. In fact, the attempt to develop a second generation vaccine against this microorganism is now possible by analyzing recombinant genomic DNA libraries ofM. tuberculosis with monoclonal antibodies and T cell clones. In the near future, the identification of epitopes recognized by mycobacterial-specific T cells with helper, cytotoxic and suppressor functions will allow the preparation of recombinant and synthetic vaccines effective in the control of this disease.
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Ameglio F., Capobianchi M. R., Dolei A., Tosi R.: Differential effects of gamma interferon on expression of HLA class II molecules controlled by the DR and DC loci — Infect. Immun.42, 122, 1983.
Andrew P. W., Rees A. D. M., Scoging A., Dobson N., Matthews R., Whittall J. T., Coates A. R. M., Lowrie D. B.: Secretion of macrophage-activating factor distinct from interferon gamma by human T cell clones — Europ. J. Immunol.14, 962, 1984.
Asherson G. L., Colizzi V., Zembala M.: An overview of T-suppressor cell circuits — Ann. Rev. Immunol.4, 37, 1986.
Chaparas S. D.: Immunity in tuberculosis — Bull. Wld Hlth Org.60, 447, 1982.
Chen-Woan M., MacGregor D. D.: The mediators of acquired resistance toListeria monocytogenes are contained within a population of cytotoxic T cells — Cell. Immunol.87, 538, 1984.
Chesnut R. W., Colon S. M., Grey H. M.: Antigen presentation by normal B cells, B cell tumors, and macrophages: functional and biochemical comparison — J. Immunol.128, 1764, 1982.
Chu E., Umetsu D., Lareau M., Schneeberger E., Geha R. S.: Analysis of antigen uptake and presentation by Epstein-Barr virus-transformed human lymphoblastoid B cells — Europ. J. Immunol.14, 291, 1984.
Colizzi V.:In vivo andin vitro administration of interleukin-2-containing preparation reverses T cell unresponsiveness inM. bovis BCG-infected mice — Infect. Immun.45, 25, 1984.
Colizzi V., Adorini L., Doria G.: Immunoregulation of lysozyme-specific suppression. I. Induction and suppression of delayed-type hypersensitivity to hen egg-white lysozyme — Europ. J. Immunol.14, 820, 1984.
Colizzi V., Asherson G. L., Malkovsky M., Garreau F., Ferluga J.: Suppressor cells induced by BCG release nonspecific factorsin vitro which inhibit DNA synthesis and IL-2 production — Immunology51, 655, 1984.
Colizzi V., Giuntini M., Garzelli C., Campa M., Falcone G.: Auto-antiidiotypic antibodies inhibit T cell-mediated hypersensitivity in BCG-infected mice — Cell. Immunol.80, 205, 1983.
Colizzi V., Malkovsky M.: Augmentation of interleukin-2 production and delayed hypersensitivity in mice infected withMycobacterium bovis and fed a diet supplemented with vitamin A acetate — Infect. Immun.48, 581, 1985.
Del Gallo F., Lombardi G., Vismara D., Piccolella E., Pizzoli P., Bufardeci E., Dolei A., Arienzo F., Gidaro M., Di Giacomo P., Colizzi V.: Effect of recombinant IL-2 on mycobacterial T cell unresponsiveness in advanced disseminated tuberculosis — Immunology. (Submitted for publication).
Emmerich F., Thole J., van Embden J., Kaufmann H. E.: A recombinant 64 kD protein ofMycobacterium bovis BCG specifically stimulates human T4 clones reactive to mycobacterial antigens — J. exp. Med.163, 1024, 1986.
Faherty D. A., Johnson D. R., Zauderer M.: Origin and specificity of autoreactive T cells in antigen-induced populations — J. exp. Med.161, 1293, 1985.
Garzelli C., Puglisi C., Falcone G.: Human monoclonal antibody to purified protein derivative produced by hybrids constructed with Epstein-Barr virus-transformed B lymphocytes and mouse myeloma cells — Europ. J. Immunol.16, 584, 1986.
Grey H. M., Colon S. M., Chesnut R. W.: Requirements for the processing of antigen by antigen-presenting B cells. II. Biochemical comparison of the fate of antigen in B cell tumors and macrophages — J. Immunol.129, 2389, 1982.
Hahn H., Kaufmann S. H. E.: The role of cell-mediated immunity in bacterial infections — Rev. infect. Dis.3, 1221, 1981.
Hank J. A., Sondel P. M.: Soluble bacterial antigen induces specific helper and cytotoxic responses by human lymphocytesin vitro — J. Immunol.128, 2734, 1982.
Kapsenberg M. L., Teunissen M. B. M., Stiekema F. E. M., Keizer H. G.: Antigen-presenting cells of dendritic cells and macrophages in proliferative T cell response to soluble and particulate antigens — Europ. J. Immunol.16, 345, 1986.
Kaufmann S. H. E.: Effective antibacterial protection induced by aListeria monocytogenes-specific T cell clone and its lymphokines — Infect. Immun.39, 1265, 1983.
Kaufmann S. H. E.: T cell clones in the study of acquired resistance to facultative intracellular bacteria. In:von Boehmer H., Haas W. (Eds): T cell clones. Elsevier Sci. Publ., Amsterdam, 1985.
Kaufmann S. H. E., Hug E., De Libero G.:Listeria monocytogenes-reactive T lymphocyte clones with cytolytic activity against infected target cells — J. exp. Med. (In press).
Kaufmann S. H. E., Simon M. M., Hahn H.: Regulatory interactions between macrophages and T cell subsets inListeria monocytogenes-specific T cell activation — Infect. Immun.38, 907, 1982.
Kaye P. M., Chain B. M., Feldmann M.: Nonphagocytic dendritic cells are effective accessory cells for anti-mycobacterial responsesin vitro — J. Immunol.134, 1930, 1985.
Lanzavecchia A.: Antigen-specific interaction between T and B cells — Nature (Lond.)314, 537, 1985.
Lombardi G., Del Gallo F., Vismara D., Piccolella E., De Martino C., Garzelli C., Puglisi C., Colizzi V.: Epstein-Barr virus-transformed B cells process and presentMycobacterium tuberculosis particulate antigens to T cells — Cell. Immunol. (In press).
Lombardi G., Di Massimo A. M., Del Gallo F., Vismara D., Piccolella E., Pugliese O., Colizzi V.: Mechanism of action of an antigen nonspecific inhibitory factor produced by human T cells stimulated by MPPS and PPD — Cell. Immunol.98, 434, 1986.
Lombardi G., Piccolella E., Vismara D., Colizzi V., Asherson G. L.:Candida albicans polysaccharide extract (MPPS) and PPD stimulate the production of IL-1 and lymphocyte proliferation — Clin. exp. Immunol.58, 581, 1984.
Lombardi G., Piccolella E., Vismara D., Colizzi V., Zembala M.: Monocyte subsets in the production of inhibitory factor byCandida albicans-activated human T cells — Immunology56, 373, 1985.
Lombardi G., Vismara D., Piccolella E., Colizzi V., Asherson G. L.: A non-specific inhibitor produced byCandida albicans-activated T cells impairs cell proliferation by inhibiting interleukin-1 production — Clin. exp. Immunol.60, 303, 1985.
Londei M., Lamb J. R., Bottazzo G. F., Feldmann M.: Epithelial cells expressing aberrant MHC class II determinants can present antigen to cloned human T cells — Nature (Lond.)312, 639, 1985.
Malynn B. A., Romeo D. T., Wortis H. H.: Antigen-specific B cells efficiently present low doses of antigen for induction of T cell proliferation — J. Immunol.135, 980, 1985.
Matthews R., Scoging A., Rees A. D. M.: Mycobacterial antigen-specific human T cell clones secreting macrophage-activating factors — Immunology54, 17, 1985.
Ministero della Sanità: Inchiesta epidemiologica sulla TBC — Circolare n. 88, 1983.
Möller G. (Ed.): T cell clones — Immunol. Rev.54, 1, 1981.
Moretta A., Pantaleo G., Lopez-Botet M., Mingari M. C., Moretta L.: Anticlonotypic monoclonal antibodies induce proliferation of clonotype-positive T cells in peripheral blood human T lymphocytes — J. exp. Med.162, 1393, 1985.
Nathan C. F., Murray H. W., Wiebe M. W., Rubin B. Y.: Identification of interferon as the lymphokine that activates human macrophages oxidative metabolism and antimicrobial activity — J. exp. Med.158, 2159, 1983.
Nathan C. F., Nakagawara A.: Role of reactive oxygen intermediates in macrophage killing of intracellular pathogens: a review. In:Mizuno D., Cohn Z. A., Takeya K., Ishida N. (Eds): Self-defence mechanism: role of macrophages. University of Tokyo Press, Tokyo, 1982; p. 279.
Onwubalili J. K., Scott G. M., Robinson J. A.: Deficient immune interferon production in tuberculosis — Clin. exp. Immunol.59, 405, 1985.
Orme I. M., Collins F. M.: Protection againstM. tuberculosis infection by adoptive immunotherapy — J. exp. Med.158, 74, 1983.
Ottenhoff T. H. M., Klatser P. R., Ivanyi J., Elferink D. G., De Wit M. Y. L., De Vries R. R. P.:Mycobacterium leprae-specific protein antigens defined by cloned human helper T cells — Nature (Lond.)319, 427, 1986.
Pennica D., Nedwin G. E., Hayflick J. S., Seeburg P. H., Derynck R., Palladino M. A., Kohr W. J., Aggarwal B. B., Goeddel D. V.: Human tumor necrosis factor: precursor, structure, expression and homology to lymphotoxin — Nature (Lond.)312, 724, 1984.
Piccolella E., Dolei A., Lombardi G., Vismara D., Pizzoli P., Colizzi V., Dianzani F.: Regulation of immune interferon production during the response to soluble microbial antigens — Clin. exp. Immunol.65, 191, 1986.
Piccolella E., Lombardi G., Morelli R.: Generation of suppressor cells in the response of human lymphocytes to a polysaccharide purified fromCandida albicans — J. Immunol.126, 2151, 1981.
Rock K. L., Benacerraf B., Abbas A. K.: Antigen presentation by hapten-specific B lymphocytes. I. Role of surface Ig receptors — J. exp. Med.160, 1102, 1984.
Ruddle N. H.: Lymphotoxin redux — Immunol. Today6, 156, 1985.
Schwartz R. H.: T lymphocyte recognition of antigen in association with gene products of the major histocompatibility complex — Ann. Rev. Immunol.3, 237, 1985.
Sinigaglia F., Pink J. R. L.: Human T lymphocyte clones specific for malaria (Plasmodium falciparum) antigens — EMBO J.4, 3819, 1985.
Thole J. E. R., Dauwerse U. G., Groothuis D. G., Schouls L. M., van Embden J. D. A.: Cloning ofMycobacterium bovis BCG DNA and expression of antigens inEscherichia coli — Infect. Immun.50, 800, 1985.
Vismara D., Lombardi G., Piccolella E., Colizzi V.: Dissociation between IL-1 and IL-2 production in proliferative response to microbial antigens: restorative effect of exogenous IL-2 — Infect. Immun.49, 298, 1985.
WHO Workshop: Characterization of antigens recognized byMycobacterium-specific monoclonal antibodies — Infect. Immun.51, 718, 1986.
Young R. A., Bloom B. R., Grosskinsky C. M., Ivanyi J., Thomas D., Davies R. W.: Dissection ofMycobacterium tuberculosis antigens using recombinant DNA — Proc. nat. Acad. Sci. (Wash.)82, 2583, 1985.
Ziegler H. K., Unanue E. R.: Decrease in macrophage antigen catabolism caused by ammonia and chloroquine is associated with inhibition of antigen presentation to T cells — Proc. nat. Acad. Sci. (Wash.)79, 175, 1982.
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These studies were supported by ‘WHO Vaccine Development Programme’,Consiglio Nazionale delle Ricerche (CNR), Roma, Italy (grant n№ 85.00875.52) andAssociazione Italiana per la Ricerca sul Cancro (AIRC) (fellowship to G. L.).
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Lombardi, G., Del Gallo, F., Vismara, D. et al. Immunology of tuberculosis: New directions in research. La Ricerca Clin. Lab. 17, 1–15 (1987). https://doi.org/10.1007/BF02909383
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DOI: https://doi.org/10.1007/BF02909383