Summary
Investigations on tumor-associated antigen in the serum of patients with bladder cancer by using monoclonal antibody Hb 7A and sandwich ELISA were carried out on 36 patients with bladder cancer (BCa group), 18 patients with other tissue tumor (OTT group) and 22 normal subjects (control group). The average OD value of Hb 7A antigen of BCa group, OTT group and control group was 0.315±0.033, 0.124±0.026 and 0.12±0.021 respectively. The OD value of Hb 7A antigen in BCa group was significantly higher than that of control group and of OTT group (P< 0.01), but there was no obvious difference between the control group and OTT group (P> 0.05). The positive detection rate in BCa group was 86% (31/36,), while detection in all 22 normal subjects and the 18 patients of OTT group yielded negative results. The results indicated that the method of using McAb Hb 7A and sandwich ELISA, characterized by high specificity and sensitivity, is of value for clinical diagnosis and follow-up of patients with bladder cancer and general survey of persons at high risk of bladder cancer.
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References
Lutzeyer W, et al. Prognostic parameters in superficial bladder cancers an analy-sis of 315 cases. J Urol 1982;127:250–2.
Huland H, et al. Long-term mitomycin C instillation after transurethral resec-tion of superficial bladder carcinomas influence on recurrence, progression and survival. J Urol 1984;132:27–9.
Brosman SA. Experience with bacillus Calmette-Guerin in patients with super-ficial bladder cancer. J Urol 1982; 128:27–30.
Lamm DL, et al. Bacillus Calmette-Guerin immunotherapy of superficial bladder cancer. J Urol 1980;124:38–42.
Prout GR, et al. Long-term fate of 90 patients with superficial bladder cancer randomly assigned to receive or not to receive thiotepa. J Urol 1983; 130: 677–80.
Umiker W. Accuracy of cytologic diag-nosis of the urinary tract. Acta Cytol 1964;8:186–91.
Chopin DK, et al. Monoclonal antibody against transitional cell carcinoma for detection of malignant urothelial cells in bladder washing. J Urol 1985;134:260–5.
Melamed MR. Flow cytometry of uri-nary bladder. Urol Clin N Am 1984;11: 599–602.
Liu BC-S, et al. Detection of oncofe-tal bladder antigen of patients with transitional cell carcinoma. J Urol 1987; 137:1258–61.
Bander NH. Monoclonal antibody: slate of the art. J Urol 1987;137:603–12.
1981:18:29–32.
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Shun-sheng, Z., Yong-chang, Z., Ren-zhao, H. et al. Clinical studies on bladder tumor-associated antigen in serum of patients with bladder cancer by using monoclonal antibody and sandwich ELISA. Journal of Tongji Medical University 10, 48–51 (1990). https://doi.org/10.1007/BF02909122
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DOI: https://doi.org/10.1007/BF02909122