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Cell-free translation of polyribosomes from detached pumpkin cotyledons: Effects of starvation and cytokinin

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Biologia Plantarum

Abstract

The effects of 6-benzylaminopurine (BAP, 5.10−5M) treatment of pumpkin cotyledons and their starvation after excision upon polysome/monosome ratio and translational capacity of polysomes in cell-free system were studied. It has been found that starvation causes a progressive polysome degradation. Polysome translation in a wheat germ cell-free proteinsynthesizing system reveals that the translation capacity of polysome preparations decreases with the time after cotyledon excision much more sharply than polysome/monosome ratio. This indicates the starvation damage in elongation steps of protein synthesis. The decrease of postribosomal supernatants activity in the system of poly(U)-directed polyphenylalanine synthesis confirms this conclusion.

BAP treatment brings about a very rapid monosome mobilization into polysomes and activation of cell-free translation of ribosome preparations which is however closely parallel to the polysome percentage in them. That means that during this initial period of BAP action only protein synthesis initiation is under BAP control. The experiments with aurintricarboxylic acid (ATA) support this idea.

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References

  • Ananiev, E., Shakirova, F. M., Klyachko, N. L., Kulaeva, O. N.: [BAP effect on polysome formation from preexisting mRNA and ribosomes.] In Russ. - Dokl. Akad. Nauk SSSR255: 508–510, 1980.

    Google Scholar 

  • Davies, E., Larkins, B. A., Knight, R. H.: Polysomes from peas. An improved method for their isolation in the absence of ribonuclease inhibitors. - Plant Physiol.50: 581–584, 1972.

    Article  PubMed  CAS  Google Scholar 

  • Marcus, A., Efron, D., Weeks, D. P.: In Methods of Enzymology30: 749–754, 1974.

    CAS  Google Scholar 

  • Klyachko, N. L., Ananiev, E., Kulaeva, O. N.: [The rapid response of protein-synthesizing machinery of detached pumpkin cotyledons to phytohormones.] In Russ. - Dokl. Akad. Nauk SSSR243: 1334–1336, 1978.

    CAS  Google Scholar 

  • Klyachko, N. L., Ananiev, E., Kulaeva, O. N.: Effect of 6-benzylaminopurine and abscisic acid on protein synthesis in isolated pumpkin cotyledons. - Physiol. vég.17: 607–617, 1979.

    CAS  Google Scholar 

  • Klyachko, N. L., Yakovleva, L. A., Kulaeva, O. N.: [Effect of 6-benzylaminopurine treatment of detached pumpkin cotyledons on the activity of protein-synthesizing system in them.] In Russ. - Fiziol. Rast.20: 1219–1223, 1973.

    CAS  Google Scholar 

  • Kulaeva, O. N.: Cytokinin action on transcription and translation in plants. - In:Guern, J., Péaud-Lenoël, C. (ed.): Metabolism and Molecular Activities of Cytokinins. Pp. 218–227. Springer-Verlag, Berlin-Heidelberg-New York 1981.

    Google Scholar 

  • Rijven, A. H. G. C.: Initiation of polyphenylalanine synthesis and the action of cytokinins in fenugreek cotyledons. - Nature252: 257–259, 1974.

    Article  PubMed  CAS  Google Scholar 

  • Roberts, B. E., Patterson, B. M.: Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA on a cell-free system from commercial wheat germ. - Proc. nat. Acad. Sci. USA70: 2330–2334, 1973.

    Article  PubMed  CAS  Google Scholar 

  • Shakirova, F. M., Conrad, K., Klyachko, N. L., Kulaeva, O. N.: [The connection between cytokinin effect on the growth of excised pumpkin cotyledons and RNA and protein synthesis in them.] In Russ. - Fiziol. Rast.29: 52–61, 1982.

    CAS  Google Scholar 

  • Szweykowska, A., Gwozdz, E., Spychala, M.: The cytokinin control of protein synthesis in plants. -In:Guern, J., Péaud-Lenoël, C. (ed.): Metabolism and Molecular Activities of Cytokinins. Pp. 212–217. Springer-Verlag, Berlin-Heidelberg-New York, 1981.

    Google Scholar 

  • Tepfer, D. A., Fosket, D. E.: Hormone-mediated translational control of protein synthesis in cultured cells ofGlycine max. - Dev. Biol.62: 486–497, 1978.

    Article  PubMed  CAS  Google Scholar 

  • Yakovleva, L. A., Klyachko, N. L., Kulaeva, O. N.: [Action of 6-benzylaminopurine on the incorporation of14C-leucine into protein in the cell-free system from detached pumpkin cotyledons.] In Russ. - Mol. Biol.11: 868–876, 1977.

    CAS  Google Scholar 

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Authors and Affiliations

  1. K. A. Timiryazev Institute of Plant Physiology, Acad. Sci. USSR, Botanicheskaya 35, 1-273, Moscow, USSR

    N. L. Klyachko, L. A. Yakovleva, F. M. Shakirova & O. N. Kulaeva

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  1. N. L. Klyachko
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  2. L. A. Yakovleva
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  3. F. M. Shakirova
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  4. O. N. Kulaeva
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Klyachko, N.L., Yakovleva, L.A., Shakirova, F.M. et al. Cell-free translation of polyribosomes from detached pumpkin cotyledons: Effects of starvation and cytokinin. Biol Plant 24, 374–380 (1982). https://doi.org/10.1007/BF02909106

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  • DOI: https://doi.org/10.1007/BF02909106

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