Abstract
Excised barley embryos were cultivated in a liquid complete medium for 48 h and then 5-fluordeoxyuridine was added. The concentrations of 5-fluordeoxyuridine differed in the range of 6 orders of magnitude (10-8 to 10-3 M). All concentrations, except 10-8 M led to a total inhibition of mitosis during 24 h. 5-fluordeoxyuridine induced chromosomal aberrations of the non-exchange type in all concentrations used. The frequencies of induced breaks increased during the interval of 12 h in whích they were followed and, in samples fixed at later intervals after the beginning of the treatment, there was a tendency to clustering of the induced fragments in some mitotic figures. The most striking feature of the effects oliowed is the relatively small dependence on the concentration used.
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Abbreviations
- FUdR:
-
5-fluordeoxyuridine
- MI:
-
mitotic index
References
Bell, S., Wolff, S.: Studies on the mechanism of the effect of fluordeoxyuridine on chromosomes. - Proc. nat. Acad. Sci. USA51: 195–202, 1964.
Cohen, S. S., Flaks, J. G., Babner, H. D., Loeb, E. F., Lichtenstein, J.: The mode of action of 5-fluorouracil and its derivatives. - Proc. nat. Acad. Sci. USA44: 1004–1012, 1958.
Gamborg, O. L., Miller, K. A., Ojima, K.: Nutritional requirements of suspension cultures of soybean root cells. - Exp. Cell Res.60: 151–168, 1968.
Giohneb, T., Lim En Se, Velemínský, J.: The effect of methyl methanesulphonate on mitosis and ohromosomes of excised barley embryos. - Biol. Plant.18: 397–400, 1976.
Heidelberger, C.: Biochemical mechanism of action of fluorinated pyrimidines. - Exp. Cell Res. Suppl.9: 462–471, 1963.
Hsu, T. C., Humphrey, R. M., Somers, C. E.: Response of Chinese hamster and L cells to 2-deoxy-5-fluorouridine and thymidine. - J. nat. Canc. Inst.32: 839–855, 1964.
Kihlman, B. A.: The production of chromatid aberrations by 5-fluordeoxyuridine alone and in combination with X-rays and 8-ethoxycaffeine. - Caryologia15: 261–277, 1962.
Kihlman, B. A.: Action of Chemicals on Dividing Cells. - Prentice-Hall, Inc. Englewood Cliffs, New Yersey 1966.
Ondřej, M., Lim En Se: The influence of pretreatment by exogenous DNA on the frequencies of aberrations induced by X-rays in barley. - Biol. Plant.18: 366–372, 1976.
Schwammenhōferová, K., Ondřej, M.: Mitotic cycle kinetics of root meristems of isolated barley embryos and intact seedlings. Labelling of nuclei by3H thymidine and its cytogenetic consequences. - Biol. Plant.20: 351–358, 1978.
Taylor, J. H., Haut, W. F., Tung, J.: Effect of fluordeoxyuridine on DNA replication, chromosome breakage and reunion. - Proc. nat. Acad. Sci. USA48: 190–198, 1962.
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Ondřej, M. Effect of different concentrations of 5-fluordeoxyuridine on mitosis and chromosomes of barley root meristems. Biol Plant 22, 348–353 (1980). https://doi.org/10.1007/BF02908980
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DOI: https://doi.org/10.1007/BF02908980