Skip to main content
SpringerLink
Log in

Protein overexport in aSaccharomyces cerevisiae mutant is not due to facilitated release of cell-surface proteins

  • Papers
  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

Saccharomyces cerevisiae strain MW11 is a temperature-sensitive mutant which exports twenty times more proteins at 37°C than parental or wild-type strains do. To understand the mechanism underlying the protein overexport in the mutant the possibility of an altered cell-wall structure leading to facilitated release of cell-surface proteins was studied. Data on calcofluor white and zymolyase sensitivities, resistance to killer 1 toxin and determination of exported acid phosphatase and invertase did not provide evidence for alterations in the cell-wall structure that could explain the protein overexport phenotype. The results were obtained in experiments when transcription of mutated gene was discontinued which permits the full expression of the protein overexport phenotype.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Alexieva K.I., Koprinarova M., Venkov P.V.: Effective selectionof Saccharomyces cerevisiae mutants that overexport proteins.Compt. Rend. Bulg. Acad. Sci. 47, 113–116 (1994).

    Google Scholar 

  • Alexieva K.I., Klis F.M., Wedler H., Wambutt R., Venkov P.V.: Identification of the essentialEPE1 gene involved in retention of secrected proteins on the cell surface ofSaccharomyces cerevisiae cells.Internat. J. Biochem. Cell Biol. 31, 903–914 (1999).

    Article  CAS  Google Scholar 

  • Belda F., Zarate V.: Isolation and characterization ofSchizosaccharomyces pombe fragile mutants.Yeast 12, 555–564 (1996).

    Article  PubMed  CAS  Google Scholar 

  • Boone C., Sommer S. S., Hensel A., Bussey H.: YeastKRE genes provide evidence for a pathway of cell wall β-glucan assembly.J. Cell. Biol. 110, 1833–1843 (1990).

    Article  PubMed  CAS  Google Scholar 

  • De Nobel J.G., Barnett J.A.: Passage of molecules through yeast cell walls: a brief essay-review.Yeast 7, 589–598 (1991).

    Article  PubMed  Google Scholar 

  • Goldstein A., Lampen J.O.: β-d-Fructofuranoside fructohydrolase from yeast.Meth. Enzymol. 42, 504–511 (1975).

    Article  PubMed  CAS  Google Scholar 

  • Haguenauer-Tsapis R.: Protein-specific features of the general secretion pathway in yeast.Meth. Enzymol. 42, 504–511 (1992).

    Google Scholar 

  • Hutchins K., Bussey H.: Cell wall receptor for yeast killer toxin: involvement of 1:6-β-d-glucan.J. Bacteriol. 153, 163–168 (1983).

    Google Scholar 

  • Ito H., Fukuda Y., Murata K., Kimura A.: Transformation of intact yeast cells treated with alkali cations.J. Bacteriol. 153, 163–168 (1983).

    PubMed  CAS  Google Scholar 

  • Klis F.M.: Cell wall assembly in yeast.Yeast 10, 851–869 (1994).

    Article  PubMed  CAS  Google Scholar 

  • Lemire J.M., Wilcocks T., Halvorson H.O., Botstain K.A.: Regulation of repressible acid phosphatase gene transcription inSaccharomyces cerevisiae.Mol. Cell. Biol. 5, 2131–3141 (1985).

    PubMed  CAS  Google Scholar 

  • Makarow M.: Secretion of invertase in mitotic yeast cell.EMBO J. 7, 1475–1482 (1988).

    PubMed  CAS  Google Scholar 

  • Maniatis X.X., Fritsch E.F., Sambrook J., inMolecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor (NY) 1982.

    Google Scholar 

  • Ram A.F.J., Wolters A., Brekelmans S.S.C., Klis F.M.: A new approach for isolating cell wall mutants ofSaccharomyces cerevisiae by screening for hypersensitivity to Calcofluor White.Yeast 10, 1019–1030 (1994).

    Article  PubMed  CAS  Google Scholar 

  • Roncero C., Duran A.: Effect of Calcofluor White and Congo Red on fungal cell wall morphogenesis:in vivo activation of chitin polymerization.Genetics 119, 499–506 (1985).

    Google Scholar 

  • Sakai A., Shimizu Y., Hishinuma F.: Isolation and characterization of mutants which show an oversecretion phenotype inSaccharomyces cerevisiae.Genetics 119, 499–506 (1988).

    PubMed  CAS  Google Scholar 

  • Smith R.A., Duncan M.J., Moir D.T.: Heterologous protein secretion from yeast.Science 29, 1219–1224 (1985).

    Article  Google Scholar 

  • Suzuki K., Ichikawa K., Jigami Y.: Yeast mutants with enhanced ability to secrete human lysozyme: isolation and identification of a protease-deficient mutant.Mol. Gen. Genet. 219, 58–64 (1989).

    Article  PubMed  CAS  Google Scholar 

  • Young T.W.: Killer yeasts, pp. 131–164 in A.H. Rose, L.S. Harrison (Eds):The Yeasts, Vol. 2. Academic Press, London 1987.

    Google Scholar 

  • Waltscheva L., Philipova D., Venkov P.: Increased extracellular secretion in fragile mutants ofSaccharomyces cerevisiae.Yeast 5, 313–320 (1989).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Molecular Biology, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria

    K. I. Alexieva & P. V. Venkov

Authors
  1. K. I. Alexieva
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. V. Venkov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to K. I. Alexieva.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Alexieva, K.I., Venkov, P.V. Protein overexport in aSaccharomyces cerevisiae mutant is not due to facilitated release of cell-surface proteins. Folia Microbiol 45, 251–254 (2000). https://doi.org/10.1007/BF02908954

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02908954

Keywords

Search

Navigation