Abstract
Half barley seeds derived from reciprocal crosses between Hiproly and the normal lysine genotypes Nordal and Welam as well as between Hiproly and CI 4362 (Hiproly isogenic sisterline) were analyzed for their content of the lysine rich chymotrypsin inhibitor named CI-2. Analysis of F1 and F2 seeds suggested that a high content of inhibitor CI-2 is inherited as a single recessive trait and confirmed the close genetic relationship between Hiproly and CI 4362. Expression of a high CI-2 content in F2 was found to be different in reciprocal crosses of Hiproly with Nordal or Welam. Screening for Hiproly segregants by dye binding was compared to analysis by the CI-2 quantification method. A good correlation was obtained and this permits the conclusion that a high CI-2 content is diagnostic for the presence of thelys gene. Quantification of CI-2 in half seeds is useful for breeding towards an increase in this lysine-rich protein.
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Abbreviations
- CI-1 and CI-2:
-
chymotrypsin inhibitor 1 and 2 from barley
- DBC:
-
dye binding capacity
References
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Jonassen, I. Segregation of high and low chymotrypsin inhibitor CI-2 content in crosses between hiproly and normal lysine barley genotypes. Carlsberg Res. Commun. 47, 305–312 (1982). https://doi.org/10.1007/BF02907792
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DOI: https://doi.org/10.1007/BF02907792