Carlsberg Research Communications

, Volume 47, Issue 5, pp 285–289 | Cite as

The primary structure of noxiustoxin: A K+ channel blocking peptide, purified from the venom of the scorpion Centruroides noxius Hoffmann

  • Lourival Domingos Possani
  • Brian M. Martin
  • IB Svendsen


Noxiustoxin, component II-11 from the venom of scorpion Centruroides noxiusHoffmann, was obtained in pure form after fractionation by Sephadex G-50 chromatography followed by ion exchange separation on carboxy-methylcellulose columns (17). The primary structure of Noxiustoxin, a polypeptide 39 amino acid residues long was determined by automaticEdman degradation and chemical cleavage with cyanogen bromide followed by amino acid analysis of the two resulting peptides. Its sequence is: Thr−Ile−Ile−Asn−Val−Lys−Cys−Thr−Ser−Pro−Lys−Gln−Cys−Ser−Lys−Pro−Cys−Lys−Glu−Leu−Tyr−Gly−Ser−Ser−Ala−Gly−Ala−Lys−Cys−Met−Asn−Gly−Lys−Cys−Lys−Cys−Tyr−Asx−Asn, with a molecular weight of 4,184±6. No histidine, arginine, tryptophan or phenylalanine was found. Noxiustoxin is the first short toxin directed against mammals and the first K+ channel blocking polypeptide-toxin (4) found in scorpion venoms.


Scorpion toxins Centruroides noxius Tityus serrulatus amino acid analysis amino acid sequence primary structure sequence homology 


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Copyright information

© Carlsberg Laboratory 1982

Authors and Affiliations

  • Lourival Domingos Possani
    • 1
  • Brian M. Martin
    • 2
  • IB Svendsen
    • 3
  1. 1.Departamento de Biologia Molecular, Instituto de Investigaciones BiomédicasUNAMMéxicoMexico
  2. 2.Max-Planck-Institut für ErnährungsphysiologieDortmundFRG
  3. 3.Department of ChemistryCarlsberg LaboratoryCopenhagen Valby

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