Abstract
The relative amounts of [1-14C]-acetate incorporated by tissue slices from primary leaves of Bonus barley into intracellular and epicuticular lipids were determined. Dark grown tissue incorporates about 2.5 times more label into intracellular fatty acids than into epicuticular lipids. Light grown tissue synthesizes the same amount of epicuticular lipids but ten times more intracellular fatty acids. The same total and relative amounts of incorporation were found when tissue slices fromcer-j 59 were studied. Furthermore, no difference in the distributions of label among saturated, monoenoic, dienoic and trienoic fatty acids from the two genotypes were detected. This supports the contention that the block incer-j 59 is independent of intracellular fatty acid synthesis.
The locuscer-j 59 blocks the production and use of C26 fatty acyl chains in the reductive pathway of epicuticular wax synthesis while increasing the relative importance of C22 chains. Wax on leaves of dark grown Bonus seedlings is a reasonable phenocopy of that present oncer-j 59 light grown ones. The sites of control of wax synthesis by the locuscer-j and light as deduced from compositional analysis are confirmed by the present tissue slice incorporation experiments with [1-14C]-C2, −C16 and −C18 precursors. Although the distribution of label among the epicuticular lipid classes was not always a facsimile of the appropriate mass distribution, the distribution of label among the primary alcohols mimicked that of the corresponding mass distribution with respect to the relative importance of the C22 and C26 chain lengths. In wax synthesized by dark grown tissue the C22 alcohol was as well or better labelled than that with 24 carbons, whereas the latter was always more heavily labelled than the former in the wax from light grown tissue.
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Abbreviations
- C∶M:
-
chloroform: methanol
- cpm:
-
counts per minute
- C2 :
-
acetate
- C16 :
-
palmitate
- C16 :
-
palmitoleate (monoenoic)
- C18 :
-
stearate
- C18 :
-
oleate (monoenoic)
- C18∶2 :
-
linoleate (dienoic)
- C18∶3 :
-
linolenate (trienoic)
- radio-GLC:
-
radio gas liquid chromatography
- TLC:
-
thin layer chromatography
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Avato, P., Mikkelsen, J.D. & von Wettstein-knowles, P. Synthesis of epicuticular primary alcohols and intracellular fatty acids by tissue slices fromcer-j 59 barley leaves. Carlsberg Res. Commun. 47, 377–390 (1982). https://doi.org/10.1007/BF02907776
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DOI: https://doi.org/10.1007/BF02907776