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A thylakoid polypeptide involved in the reconstitution of photosynthetic oxygen evolution

  • Birger Lindberg Møller
  • Peter Bordier Høj
Article

Abstract

Sonication of barley thylakoids in a high salt buffer released three polypeptides of Mr 32,000, 23,000, and 13,500 which were purified to homogeneity by chromatofocusing.

Highly purified inside-out photosystem II preparations were obtained by French Press treatment or by Triton X-100 fractionation of stacked lamellar systems. Both preparations are composed of pairs of appressed membrane sheets. In the French Press preparation, the majority of these membrane pairs are sealed whereas they are predominantly unsealed in the Triton X-100 preparation. Both preparations are able to evolve oxygen and show reversed proton pumping. The oxygen evolving capacity and variable fluorescence of both preparations were lost upon washing with high salt buffer. This treatment also removed three polypeptides at Mr 32,000, 23,000, and 13,500. The inactivated preparations were reconstituted with respect to oxygen evolution and variable fluorescence by rebinding of the isolated Mr 23,000 polypeptide. The Mr 32,000 and 13,500 polypeptides had no effect on reconstitution.

Keywords

Barley sonication chromatofocusing electron microscopy sodium dodecyl sulfate polyacrylamide gel electrophoresis variable fluorescence histones photosystem II inside-out vesicles Tris hydroxylamine 

Abbreviations

Chl

chlorophyll

chl-P

chlorophyll-protein

DCCD

dicyclohexyl carbodiimide

DCMU

3-(3,4-dichlorophenyl)-1,1-dimethylurea

DCPIP

2,6-dichlorophenol indophenol

EPR

electron paramagnetic resonance

Hepes

N-2-hydroxyethylpiperazine-N′-2-ethane sulfonic acid

PPBQ

phenyl-p-benzoquinone

PS

photosystem

SDS-PAGE

sodium dodecylsulfate polyacrylamide gel electrophoresis

TMBZ

3,3′,5,5′-tetramethylbenzidine

Tricine

N-(2-hydroxy-1,1-bis(hydroxymethyl)ethyl)glycine

Tris

tris-(hydroxymethyl)-amino methane

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Copyright information

© Carlsberg Laboratory 1983

Authors and Affiliations

  • Birger Lindberg Møller
    • 1
  • Peter Bordier Høj
    • 1
    • 2
  1. 1.Department of PhysiologyCarlsberg LaboratoryCopenhagen Valby
  2. 2.Institute of GeneticsUniversity of CopenhagenCopenhagen K

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