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Effect of antisense oligonucleotides targeting telomerase catalytic subunit on tumor cell proliferationin vitro

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Chinese Science Bulletin

Abstract

To screen specific antitumor drugs targeting telomerase catalytic subunit (hEST2), 12 differenthEST2 antisense oligonucleotides were designed based on hEST2 mRNA second structure and transfected into tumor cell lines by the lipofectin-mediated method. Cell growth activity was evaluated by MTT assay.hEST212 was picked out and its specificity, antitumor tree and continuous effect were analyzed. The results showed thathEST212 had promising antitumor activityin vitro, hEST2 can be used as a pratical target and an antisense drug candidate for cancer.

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Authors and Affiliations

  1. Beijing Institution of Radiation Medicine, 100850, Beijing, China

    Shengqi Wang, Li Lin, Zhongbin Chen, Ruxian Lin, Suhong Chen, Wei Guan & Xiaohong Wang

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  1. Shengqi Wang
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  2. Li Lin
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  3. Zhongbin Chen
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  4. Ruxian Lin
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  5. Suhong Chen
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  6. Wei Guan
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  7. Xiaohong Wang
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Correspondence to Shengqi Wang.

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Wang, S., Lin, L., Chen, Z. et al. Effect of antisense oligonucleotides targeting telomerase catalytic subunit on tumor cell proliferationin vitro . Chin. Sci. Bull. 47, 993–997 (2002). https://doi.org/10.1007/BF02907568

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  • DOI: https://doi.org/10.1007/BF02907568

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