Abstract
Potato root cultures transformed bypRiA4 T-DNA do not regenerate plants in vitro spontaneously, but regeneration can be induced by a sequence of callogenesis and regeneration media. Both Tl and Tr-DNA were found by Southern blotting. Regenerated transformants showed characteristic morphological deviations in the invitro cultures. Plants in the soil showed differences in the inflorescence and tuber development.
References
An, G.,Watson, B. D.,Stachel, S.,Gordon, M. P.,Nester, E. W.: New cloning vehicles for transformation of higher plants. - EMBO J.4: 277–284, 1985.
Cardarelli, M.,Spano, L.,Mariotti, D.,Mauro, M. L.,Van Sluyss, M. A.,Costanttno, P.: The role of auxin in hairy root induction. - Mol. gen. Genet.208: 457–463, 1987.
Huffman, G. A.,White, F. F.,Gordon, M. P.,Nester, E. W.: Hairy root inducing plasmid: physical map and homology to tumor inducing plasmids. - J. Bacteriol.157: 269–276, 1984.
Koplow, J.,Byrne, M. G.,Tempe, J.,Chilton, M. D.: Physical map of theAgrobacterium rhizogenes strain 8196 virulence plasmid. - Plasmid11: 17–27, 1984.
Murashige, T.,Skoog, F.: A revised medium for rapid growth and bioassay with tobacco tissue cultures. -Physiol. Plant.15: 473–497, 1962.
Ondřej, M.,BÍSKOVÁ, R.: Plant regeneration fromAgrobacterium rhizogenes induced tumors.- In:Procházka, S.,Hradilík, J. (ed.): Regulation of Plant Integrity. [Acta Univ. Agr.] Sb. vys. Školy zeměd. Brno, Řada A, 33. Pp. 475–477, Brno 1985.
Ondřej, M.,Prottva, J.:In vitro culture of crown gall and hairy root tumors ofAtropa belladonna. Differentiation and alkaloid production. - Biol. Plant.29: 241–246, 1987.
Ondřej, M.,Vlasák, J.: Experimental protocol of optimalization of leaf disc method ofAgrobacterium transformation for individual species of cultured plants. - Genet. Siecht. (Praha)25: 1–10, 1989.
Ooms, G.,Karp, A.,Burrell, M. M.,Twell, D.,Roberts, J.: Genetic modification of potato development using Ri T-DNA. - Theor, appl. Genet.70: 440–446, 1985.
Petit, A.,David, C.,Dahl, G. A.,Ellis, J. G.,Guyon, P.,Casse-Delbart, F.,Tempé, J.: Further extension of the opine concept: plasmids inAgrobacterium rhizogenes cooperate for opine degradation. - Mol. gen. Genet.190: 204–215, 1983.
Rogers, S. G.,Horsch, R. B.,Fraley, R. T.: Gene transfer in plants: production of transformed plants using Ri plasmid vectors. - Methods Enzymol.118: 627–640, 1986.
Webb, K. J.,Osifo, E. O.,Heshaw, G. G.: Potato tissue culture conditions. - Plant Sci. Lett.30:1–8, 1983.
White, F. F.,Taylor, B. H.,Huffman, G. A.,Gordon, M. P.,Nester, E. W.: Molecular and genetic analysis of the transferred DNA region of the root-inducing plasmid ofAgrobacterium rhizogenes. - J. Bacteriol.164: 33–44, 1985.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ondřej, M., Hrouda, M. & Kostřica, P. Potato transformation byAgrobacterium rhizogenes Ri plasmid. Biol Plant 31, 312–314 (1989). https://doi.org/10.1007/BF02907293
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02907293