Abstract
Recurrent application (17 administrations in 38 days) of 14 pesticides (Karathane FN 57, Fundazol 50 WP, Dithane M 45, Topsin M 70 WP, Perozin 75 B, Fademorf EK 20, Novozir MN 80, Metation E 50, Pirimor DP, Decemtion EK 20, Zeazin 50 DP, Fatex EK 80, maleic hydrazide and Ethrel) did not increase (with the exception of maleic hydrazide) the frequency of somatic mutations in a heterozygous chlorophyll mutant ofNicotiana tabacumL. var.xanthi n. c. None of the four tested pesticides (Fademorf EK 20, Decemtion EK 20, maleic hydrazide and Ethrel) were transformed in tobacco plants to a stable mutagenic or promutagenic product active in theSalmonella (Ames) mutagenicity assay.
Similar content being viewed by others
References
Ames, B. N., Durston, W. E., Yamasaki, E., Lee, F. D.: Carcinogens are mutagens: a simple test system combining liver homogenates for activation and bacteria for detection. - Proc. nat. Acad. Sci. USA70: 2281–2285, 1973.
Bříza, J..,Gichner, T., Velemínský, J.: Somatic mutations in tobacco plants after hronic exposure to maleic hydrazide and its diethanolamine and potassium salts. - Mutation Res.139: 25–28, 1984.
Bříza, J., Gichner, T., Velemínský, J., Opatrný, Z.: Mutational events after recurrent or chronic exposures of mutagenic and promutagenic N-nitroso compounds to a heterozygous tobacco tester strain. - Biol. Plant.27: 22–27, 1985.
Dulieu, H. L., Dalebroux, M. A.: Spontaneous and induced reversion rates in a double heterozygous mutant ofNicotiana tabaccum var. xanthi n. c.; dose-response relationship. - Mutation Res.30: 63–70, 1975.
Fabries, M., Delpoux, M.: Genetic effects of low and very low chronic doses of gamma-irradiation on the a1 + /a1 a2 +/a2 system of tobacco. - Mutation Res.49: 377–382, 1978.
Gentile, J. M., Gentile, G. J., Bultman, J., Sechriest, R., Wagner, E. D., Plewa, M. J.: An evaluation of the genotoxic properties of insecticides following plant and animal activation. -Mutation Res.101: 19–29, 1982.
Gentile, J. M., Pluymers, D., Plewa, M. J.: Promutagen activation as a function of growth curve dynamics in the plant cell/microbe coincubation assay. - Mutation Res.173: 181–185, 1986.
Gentile, J. M., Wagner, E. D., Plewa, M. J.: The detection of weak recombinogenic activities in the herbicides alachlor and propachlor using a plant-activation bioassay. - Mutation Res.48: 113–116, 1977.
Georgian, L., Moraru, I., Draghicescu, T., Dinu, I., Ghizelea, G.: Cytogenetic effects of alachlor and mancozeb. - Mutation Res.116: 341–348, 1983.
Gichner, T., Velemínský, J., Pánková, K.: Differential response to three alkylating nitrosocompounds and three agricultural chemicals in theSalmonella (Ames) and in theTradescantia, Arabidopsis and barley mutagenicity assays. - Biol. Zentralbl.101: 375–383, 1982a.
Gichner, T., Velemínský, J., Pokorný, V.: Somatic mutations induced by maleic hydrazide and its potassium and diethanolamine salts in theTradescantia mutation assay. - Mutation Res.103: 289–293, 1982b.
Kappas, A., Green, M. H. L., Bridges, B. A., Rogers, A. M., Muriel, W. J.: Benomyl — a novel type of base analogue mutagen? - Mutation Res.40: 379–382, 1976.
Pilinskaya, M. A.: [Investigation of cytogenetic effects of pesticide captan and benomyl in the culture of human peripheral lymphocytes invitro with and without metabolic activation.] In Russ. - Tsitol. Genet.17: 30–34, 1983.
Plewa, M. J., Gentile, J. M.: Mutagenicity of atrazine: a maize-microbe bioassay. - Mutation Res.38: 287–292, 1976.
Plewa, M. J., Gentile, J. M.: The activation of chemicals into mutagens by green plants. - In:De Serres, F. J., Hollaender, A. (ed.): Chemical Mutagens: Principles and Methods for Their Detection. Vol.7. Pp. 401–420. Plenum Press, New York-London 1982.
Plewa, M. J., Wagner, E. D.: Germinal cell mutagenesis in specially designed maize genotypes. - Environ. Health Perspect.37: 61–73, 1981.
Plewa, M. J., Wagner, E. D., Gentile, G. J., Gentile, J. M.: An evaluation of the genotoxic properties of herbicides following plant and animal activation. - Mutation Res.136: 233–245, 1984.
Sakamoto, E. T., Takahashi, C. S.: Action of benlate fungicide onTradescantia stamen hairs andAllium cepa root-tip cells. - Rev. brasil. Genet.4: 367–381, 1981..
Velemínský, J., Gichner, T.: Mutagenic activity of promutagens in plants: indirect evidence of their activation. - Mutation Res.197: 221–242, 1988.
Yahagi, T., Degawa, M., Seino, Y., Matsushima, T., Nagao, M., Sugimura, T., Hashimoto, Y.: Mutagenicity of carcinogenic azo dyes and their derivatives. - Cancer Lett.1: 91–96, 1975.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Břĺza, J. Estimation of mutagenicity and metabolic activation after recurrent exposures ofnicotiana tabacum L. var.xanthi to 14 pesticides. Biol Plant 31, 145–151 (1989). https://doi.org/10.1007/BF02907248
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02907248