Abstract
Bromegrass cell suspension cultures, varying in frost hardiness, were immersed ìn distilled water and electrical conductivity of the bathing solutions was measured at various intervals after immersion. It was found that tissue frost-hardened by maintenance at 2‡C leaked more electrolytes than the relatively frost-sensitive tissue grown at 20‡C, but that tissue frost-hardened by abscisic add treatment displayed decreased leakage, despite comparable levels of frost-hardiness having been achieved. It is concluded, in contrast with the conclusion of a previous report, that changes in membrane permeability to electrolytes may not be directly related to the capacity of cells to tolerate freezing stress.
Similar content being viewed by others
References
Chen, T. H. H., Gusta, L. V.: Abscisic acid-induced freezing resistance in cultured plant cells. - Plant Physiol.73 :71–75, 1983.
Dexter, S. Tottinggham, W. E., Graber, L. F.: Investigations of hardiness of plants by measurement of electrical conductivity. - Plant Physiol.7 :63–78, 1932.
Kacpbrska-Palacz, A., Dlugokecka, E., Breitenwald, J., Wciślińska, B.: Physiological mechanisms of frost tolerance : possible role of protein in plant adaptation to cold. - Biol. Plant.19 : 10–17, 1977.
Palta, J. P., Levitt, J., Stadelmann, E. J.: Freezing injury in onion bulbs. I. Evaluation of the conductivity method and ion and sugar efflux from injured cells. - Plant Physiol.60: :393–397, 1977.
Piotrowska, G., Kacperska, A.: Relationship between ATP content and desiccation-induced injuries in winter rape hypocotyls. - J. Plant Physiol.128 :485–490, 1987.
Pukacki, P., Pukacka, S.: Freezing stress and membrane injury of Norway spruce (Picea ab/es) tissues. -Physiol. Rant.69 :156–160, 1987.
Raymond, A., Harwood, C. E., Owen, J. V.: A conductivity method for screening populations of eucalypts for frost damage and frost tolerance. - Aust. J. Bot.34 : 377–393,1986.
Sukumaran, N. P., Weiser, J.: An excised leaflet test for evaluating potato frost tolerance. - HortScience7: 467–468, 1972.
vanSwaaij, A. C., Talsma, Krugsheld, H., Jacobsen, E., Feenatra, W. J.: Frost tolerance in cell cultures of potato. - Physiol. Plant.69 : 602–608, 1987.
Zhang, M. I. N.: Ultrastructural observations of the plasma membrane of bromegrass (Bromus inenais Leyss) cell suspension culture during frost hardening—a freeze-fracture approach. - M. Sc. Thesis, Dalhousie University, Halifax 1986.
Zhang, M. I. N., Willison, J. H. M.: Ultrastructure of the cell wall of bromegrass (Bromus inermis Leyss) cell suspension culture. - Plant Cell Rep.5 : 448–451, 1986.
Zhang, M. I. N., Willison, J. H. M.: An improved conductivity method for the measurement of frost hardiness. -Can. J. Bot.65 : 710–715, 1987.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Zhang, M.I.N., Willison, J.H.M. Membrane permeability to electrolytes in relation to frost hardiness in bromegrassBromus inennis Leyss) Cell Suspension Culture. Biol Plant 31, 88–91 (1989). https://doi.org/10.1007/BF02907237
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02907237