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Membrane permeability to electrolytes in relation to frost hardiness in bromegrassBromus inennis Leyss) Cell Suspension Culture

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Biologia Plantarum

Abstract

Bromegrass cell suspension cultures, varying in frost hardiness, were immersed ìn distilled water and electrical conductivity of the bathing solutions was measured at various intervals after immersion. It was found that tissue frost-hardened by maintenance at 2‡C leaked more electrolytes than the relatively frost-sensitive tissue grown at 20‡C, but that tissue frost-hardened by abscisic add treatment displayed decreased leakage, despite comparable levels of frost-hardiness having been achieved. It is concluded, in contrast with the conclusion of a previous report, that changes in membrane permeability to electrolytes may not be directly related to the capacity of cells to tolerate freezing stress.

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References

  1. Chen, T. H. H., Gusta, L. V.: Abscisic acid-induced freezing resistance in cultured plant cells. - Plant Physiol.73 :71–75, 1983.

    PubMed  CAS  Google Scholar 

  2. Dexter, S. Tottinggham, W. E., Graber, L. F.: Investigations of hardiness of plants by measurement of electrical conductivity. - Plant Physiol.7 :63–78, 1932.

    PubMed  CAS  Google Scholar 

  3. Kacpbrska-Palacz, A., Dlugokecka, E., Breitenwald, J., Wciślińska, B.: Physiological mechanisms of frost tolerance : possible role of protein in plant adaptation to cold. - Biol. Plant.19 : 10–17, 1977.

    Article  Google Scholar 

  4. Palta, J. P., Levitt, J., Stadelmann, E. J.: Freezing injury in onion bulbs. I. Evaluation of the conductivity method and ion and sugar efflux from injured cells. - Plant Physiol.60: :393–397, 1977.

    CAS  Google Scholar 

  5. Piotrowska, G., Kacperska, A.: Relationship between ATP content and desiccation-induced injuries in winter rape hypocotyls. - J. Plant Physiol.128 :485–490, 1987.

    CAS  Google Scholar 

  6. Pukacki, P., Pukacka, S.: Freezing stress and membrane injury of Norway spruce (Picea ab/es) tissues. -Physiol. Rant.69 :156–160, 1987.

    CAS  Google Scholar 

  7. Raymond, A., Harwood, C. E., Owen, J. V.: A conductivity method for screening populations of eucalypts for frost damage and frost tolerance. - Aust. J. Bot.34 : 377–393,1986.

    Article  Google Scholar 

  8. Sukumaran, N. P., Weiser, J.: An excised leaflet test for evaluating potato frost tolerance. - HortScience7: 467–468, 1972.

    Google Scholar 

  9. vanSwaaij, A. C., Talsma, Krugsheld, H., Jacobsen, E., Feenatra, W. J.: Frost tolerance in cell cultures of potato. - Physiol. Plant.69 : 602–608, 1987.

    Article  Google Scholar 

  10. Zhang, M. I. N.: Ultrastructural observations of the plasma membrane of bromegrass (Bromus inenais Leyss) cell suspension culture during frost hardening—a freeze-fracture approach. - M. Sc. Thesis, Dalhousie University, Halifax 1986.

    Google Scholar 

  11. Zhang, M. I. N., Willison, J. H. M.: Ultrastructure of the cell wall of bromegrass (Bromus inermis Leyss) cell suspension culture. - Plant Cell Rep.5 : 448–451, 1986.

    Article  Google Scholar 

  12. Zhang, M. I. N., Willison, J. H. M.: An improved conductivity method for the measurement of frost hardiness. -Can. J. Bot.65 : 710–715, 1987.

    CAS  Google Scholar 

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Zhang, M.I.N., Willison, J.H.M. Membrane permeability to electrolytes in relation to frost hardiness in bromegrassBromus inennis Leyss) Cell Suspension Culture. Biol Plant 31, 88–91 (1989). https://doi.org/10.1007/BF02907237

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  • DOI: https://doi.org/10.1007/BF02907237

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