Abstract
Accuracy of activity of ribosome isolated from UV-light-induced streptomycin-resistant R-21 mutant ofStreptomyces granaticolor was measured in anE. coli-derived system translating poly(U) with a high rate and accuracy. Ribosomes from the R-21 mutant strain were shown to be resistant to streptomycin and about two-fold more accurate than those from the wild type. The mutant strain was found to be resistant to 1000 mg/L streptomycin (Stm) during vegetative growth while it sporulated on agar plates containing only up to 200 mg/L of Stm. The growth rate of the R-21 mutant in complex liquid medium was indistinguishable from that of the wild-type strain.
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Weiser, J., Ehrenberg, M. & Náprstek, J. In vitro measurement of translation accuracy of ribosomes isolated from streptomycin-resistant mutant ofStreptomyces granaticolor . Folia Microbiol 39, 129–132 (1994). https://doi.org/10.1007/BF02906807
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DOI: https://doi.org/10.1007/BF02906807