Abstract
A rapid method for the differentiation of hemolytic staphylococci is described. Instead of a β-hemolysin monoproducingStaphylococcus culture, a test strip, soaked in a stabilized product of theS. aureus strain CCM 6188 with defined cytolytic activity, is used. The results of the rapid method can be read one day earlier than those of the conventional method. A set of 137 strains ofS. aureus from various sources, including 46 enterotoxin-producing (SE-positive) ones, were examined by both methods. A higher proportion of α- and σ-hemolysin-producing strains was found among the SE-positive strains, while (α+β)-hemolysin production prevailed among the SE-negative ones.
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Adamczyk B., Blaurock G.: Zum nachweis spezifischen Staphylokokken-hämolysine.Z. Ges. Hyg.9, 456–571 (1963).
Elek S.D., Levy E.: Distribution of hemolysins in pathogenic and non-pathogenic staphylococci.J. Path. Bact.62, 541–553 (1950).
Hájek V., Maršálek E.: The differentiation of pathogenic staphylococci and a suggestion for their taxonomic classification.Zbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. I Orig.A217, 176–182 (1971).
Jassim S., Salt W.G., Stretton R.J.:In vitro studies of haemolysis by some staphylococci grown in chemically defined media.J. Appl. Bacteriol.67, 511–520 (1989).
Kloos W.E., Schleifer K.H.: Genus IV. Staphylococcus Rosenbach 1884, pp. 1013–1035 inBergey's Manual of Systematic Bacteriology, Vol. 2 (P.H.A. Sneath, Ed.). Williams & Wilkins, Baltimore 1986.
Růžičková V.: Recent knowledge of biotypes ofStaphylococcus aureus strains isolated in dairy farms. (in Czech)Veter. Med.36, 393–399 (1991).
Sharma B.S., Haque R.: A chemically defined medium for the production of staphylococcal beta haemolysin.Can. J. Microbiol.19, 1319–1323 (1973).
Wiseman G.M.: The haemolysins ofStaphylococcus aureus.Bact. Rev.39, 317–344 (1975).
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Růžičková, V. A rapid method for the differentiation ofStaphylococcus aureus hemolysins. Folia Microbiol 39, 112–114 (1994). https://doi.org/10.1007/BF02906804
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DOI: https://doi.org/10.1007/BF02906804