Abstract
We describe the transfer, at low frequency, of a limited number of nuclear markers duringkarl mediated cytoduction of the RHO+ factor. By selection for a chromosome III marker inKAR1 HIS4[RHO+]×karl his4 [rho−] crosses, strains disomic for chromosome III were isolated. Markers carried on five other chromosomes in theHIS4 donating strain could be shown to be absent from the disomic strains. When these disomic strains were force-mated to haploid tester strains the rate prototrophic products were sporulators with good spore viability. These observations suggest that only one or possibly a few chromosomes were transferred to the recipient strain during cytoduction of the RHO+ factor.
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Abbreviations
- SD:
-
synthetic minimal dextrose
- YPD:
-
yeast extract peptone dextrose
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Nilsson-Tillgren, T., Petersen, J.G.L., Holmberg, S. et al. Transfer of chromosome III duringkar mediated cytoduction in yeast. Carlsberg Res. Commun. 45, 113–117 (1980). https://doi.org/10.1007/BF02906512
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DOI: https://doi.org/10.1007/BF02906512