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The amino terminal sequence of several toxins from the venom of the Mexican scorpion Centruroides noxius Hoffmann

  • Lourival Domingos Possani
  • Myrna A. R. Dent
  • Brian M. Martin
  • Alfred Maelicke
  • Ib Svendsen
Article

Abstract

The soluble venom from the Mexican scorpion Centruroides noxiusHoffmann was fractionated by Sephadex G-50 chromatography followed by ion exchange separation on carboxymethylcellulose and Bio-Rex 70 columns. Six homogeneous toxins were obtained by this procedure. The amino acid composition and the amino-terminal amino acid sequence was determined for four of them. They are all basic polypeptides with a molecular weight in the order of 7,000, containing from 59 to 65 amino acid residues with four disulphide bridges.

Toxin II.9.2.2 has the N-terminal sequence: Lys-Glu-Gly-Tyr-Leu-Val-Asp-Lys-Asn-Thr-Gly-Cys-Lys-Tyr-Glu-Cys-Leu-Lys-Leu-Gly-Asp-Asn-Asp-Tyr-Cys-Leu-Arg-Glu-Cys-Lys-Gln-Gln-Gly-Tyr-Lys-Gly-Ala-Gly-Gly-Tyr-Cys-Tyr-Ala-Phe-Ala-Cys-Trp-Cys.

The N-terminal sequence of toxin II-10 was shown to be: Lys-Glu-Gly-Tyr-Leu-Val-Asn.

For Toxin II-13 the amino acid N-terminal sequence is: Lys-Glu-Gly-Tyr-Ile-Val-Asp-Tyr-His-Asp-Gly-Cys-Lys-Tyr-X-Cys-Tyr-Lys-Leu-Gly-Asp-Asn-Asp-Tyr, and Toxin II-14 has the amino terminal sequence: Lys-Asp-Gly-Tyr-Leu-Val-Asp-Ala-Lys-Gly-Cys-Lys-Lys-Asn-Cys-Tyr-Lys-Leu-Gly-Lys-Asn-Asp-Tyr-Cys-Asn-Arg-Glu-Cys-Arg-Met-Lys-His-Arg-Gly. Some of the biological properties of these toxins are discussed in the communication.

Keywords

Scorpion toxins Centruroides noxius amino acid analysis amino acid sequence sequence homology 

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Copyright information

© Carlsberg Laboratory 1981

Authors and Affiliations

  • Lourival Domingos Possani
    • 1
    • 2
  • Myrna A. R. Dent
    • 1
    • 2
  • Brian M. Martin
    • 2
  • Alfred Maelicke
    • 2
  • Ib Svendsen
    • 3
  1. 1.Instituto de Investigaciones BiomédicasUniversidad Nacional Autónoma de MexicoMéxico 20Mexico
  2. 2.Max-Planck-Institut für ErnährungsphysiologieDortmund
  3. 3.Department of ChemistryCarlsberg LaboratoryCopenhagen, Valby

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