Skip to main content
SpringerLink
Log in

Alterations of both responder T cells and stimulator non-T cells are responsible for abnormal mixed lymphocyte reaction in aged humans

  • Original Contributions
  • Published:
Ricerca in clinica e in laboratorio

Summary

The autologous and allogeneic mixed lymphocyte reactions of 15 young and 15 aged human adults were compared. Both autologous and allogeneic mixed lymphocyte reactions were significantly reduced in the aged group. T cells from aged adults displayed a reduced proliferative response to non-T cells of either aged or young adults. T cells from young adults also showed a reduced proliferative response to non-T cells from aged adults. Sera from aged adults, showing depression of autologous and allogeneic mixed lymphocyte reaction, did not exert any inhibitory effect on the autologous and allogeneic mixed reaction of lymphocytes from young donors. These data suggest that depression of mixed lymphocyte reaction in aged humans probably reflects intrinsic abnormalities of both responder T cells and stimulatory non-T cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Ellis T. M., Mokanakur T.: Dissociation of autologous and allogeneic mixed lymphocyte reactivity by using a monoclonal antibody specific for human helper T cells—J. Immunol.131, 2323, 1983.

    PubMed  CAS  Google Scholar 

  2. Fernandez L. A., MacSween J. M.: Decreased autologous mixed lymphocyte reaction with aging—J. Mech. Aging Develop.12, 245, 1980.

    Article  CAS  Google Scholar 

  3. Fournier C., Charriere J.: Autologous mixed lymphocyte reaction in man. II. Autoreactive and alloreactive cells belong to two different T cell subsets—J. Immunol.128, 2698, 1982.

    PubMed  CAS  Google Scholar 

  4. Hallgren H. M., Kersey J. H., Dubey D. P., Yunis E. J.: Lymphocyte subsets and integrated immune function in aging humans—Clin. Immunol. Immunopathol.10, 65, 1978.

    Article  PubMed  CAS  Google Scholar 

  5. Huber C., Fink U., Leibold W., Schmalzl F., Peterson P. A., Klareskog L., Braunsteiner H.: The role of adherent HLA-DR+ mononuclear cells in autologous and allogeneic MLR. —J. Immunol.127, 726, 1981.

    PubMed  CAS  Google Scholar 

  6. Indiveri F., Pierri I., Viglione D., Pende D., Russo C., Pellegrino M. A., Ferrone S.: Human T lymphocytes in aging and malignancy: abnormalities in PHA-induced la antigen expression and in functional activity in autologous and allogeneic MLR—Cell. Immunol.76, 224, 1983.

    Article  PubMed  CAS  Google Scholar 

  7. Kay M. B., Makinodan T.: Relationship between aging and the immune system—Progr. Allergy29, 134, 1981.

    CAS  Google Scholar 

  8. Kishimoto S., Tomino S., Inomata K., Kotegawa S., Saito T., Kuroki M., Mitsuya H., Hisamitsu S.: Age-related changes in the subsets and functions of human T lymphocytes. —J. Immunol.121, 1773, 1978.

    PubMed  CAS  Google Scholar 

  9. Kozak R. W., Moody C. E., Staiano-Loico L., Weksler M. E.: Lymphocyte transformation induced by autologous cells. XII. Quantitative and qualitative differences between human autologous and allogeneic reactive T cell subsets—J. Immunol.128, 1723, 1982.

    PubMed  CAS  Google Scholar 

  10. Moody C. E., Imes J. B., Staiano-Loico L., Incefy G. S., Thaler H. T., Weksler M. E.: Lymphocyte transformation induced by autologous cells. XI. The effect of age on the autologous mixed lymphocyte reaction—Immunology44, 431, 1981.

    PubMed  CAS  Google Scholar 

  11. Okudaira K., Searles R. P., Goodwin J. S., Williams R. C. Jr.: Antibodies in the sera of patients with systemic lupus erythematosus that block the binding of monoclonal anti-Ia to Ia-positive targets also inhibit the autologous mixed lymphocyte response—J. Immunol.129, 582, 1982.

    PubMed  CAS  Google Scholar 

  12. Palacios R., Claesson L., Möller G., Peterson P. A., Möller E.: The α-chain, not the β-chain of HLA-DR antigens participates in the activation of T cells in autologous mixed lymphocyte reaction—Immunogenetics15, 341, 1982.

    Article  PubMed  CAS  Google Scholar 

  13. Romagnani S., Giudizi M. G., Almerigogna F., Ricci M.: Interaction of staphylococcal protein A with membrane components of IgM and/or IgD-bearing lymphocyte from human tonsil—J. Immunol.124, 1620, 1980.

    PubMed  CAS  Google Scholar 

  14. Romagnani S., Rossi-Ferrini P. L., Ricc: M.: The immune derangement in Hodgkin’s disease —Semin. Haematol. 1984. (In press).

  15. Smith J. B., Talal N.: Significance of self-recognition and interleukin-2 for immunoregulation, autoimmunity and cancer—Scand. J. Immunol.16, 269, 1982.

    Article  PubMed  CAS  Google Scholar 

  16. Smolen J. S., Luger T. A., Chused T. M., Steinberg A. D.: Responder cells in the human autologous mixed lymphocyte reaction—J. clin. Invest.68, 1501, 1981.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Cattedra di Immunologia Clinica, Università degli Studi di Firenze, Viale Morgagni, 50134, Firenze, Italia

    Maria Grazia Giudizi, Fabio Almerigogna, Roberta Biagiotti, Anna Alessi & Sergio Romagnani

  2. Ospedale i Fraticini (INRCA), Viale Morgagni, 50134, Frienze, Italia

    Paola Paoli & Carlo Colonna

Authors
  1. Maria Grazia Giudizi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Fabio Almerigogna
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Roberta Biagiotti
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Anna Alessi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Paola Paoli
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Carlo Colonna
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Sergio Romagnani
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This work was supported by a grant from theConsiglio Nazionale delle Ricerche (CNR), Roma, Italy ‘Progetto Finalizzato Medicina Preventiva, Sottoprogetto Meccanissmi di Invecchiamento’.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Giudizi, M.G., Almerigogna, F., Biagiotti, R. et al. Alterations of both responder T cells and stimulator non-T cells are responsible for abnormal mixed lymphocyte reaction in aged humans. La Ricerca Clin. Lab. 14, 615–620 (1984). https://doi.org/10.1007/BF02906300

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02906300

Key-words

Search

Navigation