Summary
A histochemical study was carried out on orcein-positive hepatocellular material in 111 cases, 27 of which were stained positively by orcein. Orcein-positive material was very frequently found in protracted viral hepatitis and in chronic active hepatitis, as well as in other liver diseases with or without cholestasis; it was absent in liver cirrhosis. In all the cases considered the orcein-positive material was mainly formed of proteins, rich in amino acids with sulfhydryl and disulphide radicals, tryptophan and, to a lesser extent, of NH2 α amino acids. The orcein-positive substance was apparently carbohydrate-free. The presence of copper was also confirmed.
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References
Adams C. W. M.: Ap-dimethylaminobenzaldehyde nitrite method for the histochemical demonstration of tryptophan and related compounds—J. clin. Path.10, 56, 1957.
Adams C. W. M., Sloper J. C.: The hypothalamic elaboration of posterior pituitary principles in man, rat and dog. Histochemical evidence derived from a performic acid-alcian blue reaction for cystine—J. Endocr.13, 221, 1956.
Barka T., Anderson P.: Histochemistry—Hoeber Medical Division, New York, 1963.
Barnett R. J.: The histochemical demonstration of sites of protein-bound sulphydryl and disulphide groups—Anat. Rec.115, 280, 1953.
Barnett R. J., Seligman A. M.: Histochemical demonstration of sulphydryl and disulphide groups of proteins—Science116, 323, 1952.
Barnett R. J., Seligman A. M.: Histochemical demonstration of protein-bound sulphydryl groups—J. nat. Cancer Inst.14, 769, 1953.
Barnett R. J., Seligman A. M.: Histochemical experiments on sulphydryl and disulphide groups in glutathione—Academic Press, Inc., New York, 1954.
Bensley R. R., Gersh I.: Studies on cell structure by the freezing-dry method. Nature of the mitochondria in the hepatic cells ofAmblystoma—Anat. Rec.57, 217, 1933.
Gomori G.: Microscopic histochemistry—Chicago University Press, Chicago, 1952.
Kaufmann B. P., Gay H., McDonald M. R.: Cold. Spr. Harb. Symp. quant. Biol.14, 85, 1950 —In:Pearse A. G. E. (Ed.) Histochemistry. J. and A. Churchill, London, 1960; p. 624.
Lev R., Spichr S.: In:Lillie, R. D.: Histopathologic technic and practical histochemistry —Blakiston, New York, 1954; 2nd ed.
Lison L.: Histochimie et cytochimie animales—Gauthier-Villars, Paris, 1953.
Mazia D., Brewer P., Alfert M.: The cytochemical staining and measurement of proteins with mercuric bromophenol blue—Biol. Bull.104, 57, 1953.
Pearse A. G. E. (Ed.): Histochemistry—J. and A. Churchill, London, 1960.
Shikata T., Uzawa T., Yoshiwara N., Akatsuka T., Yamazaki S.: Staining methods of Australia antigen in paraffin sections—Jap. J. exp. Med.44, 25, 1974.
Sipponen P.: Orcein-positive hepatocellular material in long-standing biliary diseases. Histochemical characteristics—Scand. J. Gastroent.11, 545, 1976.
Yasuma A., Itchikawa T.: Ninhydrin-Schiff and alloxan-Schiff staining. A new histochemical stain method for protein—J. Lab. clin. Med.41, 296, 1953.
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Matturri, L., Cazzola, P. Histochemical study of orcein-positive hepatocellular material in paraffin sections of liver biopsy samples in the course of various liver diseases. La Ricerca Clin. Lab. 9, 41–46 (1979). https://doi.org/10.1007/BF02905026
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DOI: https://doi.org/10.1007/BF02905026