Summary
Platelet antibody determination by the PF3 test was carried out in 96 thrombocytopenic patients with various disorders, 31 repeatedly transfused patients with or without thrombocytopenia and 24 patients with autoimmune disease (SLE andmyasthenia gravis) without thrombocytopenia. The frequency of a positive test was greatest in the patients with ITP (61%), SLE (50%) or a history of numerous blood transfusions (60%). The patients withmyasthenia gravis also showed a considerable frequency (20%) of platelet antibodies detectable by the PF3 test. The PF3 test is less sensitive than the serotonin release test in detecting autoantibodies, but it is more sensitive than aggregometry in detecting isoantibodies and drug-related antibodies.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Clancy R., Jenkins E., Firkin B.: Qualitative platelet abnormalities in idiopathic thrombocytopenic purpura—New Engl. J. Med.296, 622, 1972.
David J. L.: Platelet factor 3 availability and kinetics of platelet aggregation—Acta med. scand.525 (Suppl.), 93, 1970.
De Gaetano G., Vermylen J., Verstraete M.: Platelet aggregation by specific human immunoglobulin preparation—Thrombos. Diathes, haemorrh. (Stuttg.)24, 419, 1970.
Deykin D., Hellerstein L. J.: The assessment of drug-dependent and isoimmune antiplatelet antibodies by the use of platelet aggregometry—J. clin. Invest.51, 3142, 1972.
Dixon R. H., Rosse F. W.: Platelet antibody in autoimmune thrombocytopenia—Brit. J. Haemat.31, 129, 1975.
Donnel R. L., McMillan R., Yelenosky R. J., Longmire S., Lightsey A. L.: Different antiplatelet antibody specificities in immune thrombocytopenic purpura—Brit. J. Haemat.34, 147, 1976.
Gandolfo G. M., Afeltra A., Mannella E.: La dimostrazione degli anticorpi antipiastrine nella trombocitopenia autoimmune con il test della liberazione della serotonina marcata— Folia Allerg. Immunol. clin.23, 357, 1976.
Gandolfo G. M., Afeltra A., Mannella E., Costantini G., Angeloni P.: Determinazione degli isoanticorpi antipiastrine con il test della liberazione della14C serotonina nei soggetti affetti da morbo di Cooley—Progr. med. (Napoli)33, 33, 1977.
Gockerman J. P., Bowman R. P., Conrad M. E.: Detection of platelet isoantibodies by3H serotonin platelet release and its clinical application to the problem of platelet matching— J. clin. Invest.55, 75, 1975.
Goebel K. M., Hahn E., Havemann K.: HLA matching in autoimmune thrombocytopenic purpura—Brit. J. Haemat.35, 341, 1977.
Hirschman R. J., Shulman N. R.: The use of platelet serotonin release as a sensitive method for detecting antiplatelet antibodies and a plasma antiplatelet factor in patients with idiopathic thrombocytopenic purpura—Brit. J. Haemat.24, 793, 1973.
Hirschman R. J., Yankee R. A., Coller B. S., Gralnick H. R.: Sensitive method for the detection and characterization of platelet isoantibodies—Thrombos. Diathes. haemorrh. (Stuttg.)29, 408, 1973.
Karpatkin S., Strick N., Karpatkin M. B., Siskind G. W.: Cumulative experience in the detection of antiplatelet antibody in 234 patients with idiopathic thrombocytopenic purpura, systemiclupus erythematosus and other clinical disorders—Amer. J. Med.52, 776, 1972.
Karpatkin S., Strick N., Siskind G. W.: Detection of splenic antiplatelet antibody synthesis in idiopathic autoimmune thrombocytopenic purpura (ATP)—Brit. J. Haemat.26, 167, 1972.
Okuno T., Crockatt D.: Antiplatelet antibody determination by platelet factor 3 assay— Amer. J. clin. Path.66, 475, 1976.
Pogliani E., Lambertenghi Deliliers M., Ferrari R., Pazzoli M., Cofrancesco E., Praga C.: Platelet aggregometry and platelet isoantibodies—Haemostasis4, 23, 1975.
Poole G., Shandring P., Wolledge S.: Potentially serious side effect of high dose twice-weekly rifampicin—Brit. med. J.iii, 343, 1971.
Probst E., Lüscher E. F.: Studies on thrombostenin-A, the actin-like moiety of the contractile protein from blood platelets. I. Isolation, characterization and evidence for two forms of thrombostenin—Biochim. biophys. Acta (Amst.)278, 577, 1972.
Wu K. K., Hoak J. C., Thompson J. S., Koepke J. A.: Use of platelet aggregometry in selection of compatible platelet donors—New Engl. J. Med.292, 130, 1975.
Wu K. K., Thompson J. S., Koepke J. A., Hoak J. C., Flink R.: Heterogeneity antibody response to human platelet transfusion—J. clin. Invest.58, 432, 1976.
Zarecensky S. E., Dvilansy J.: Determinación de anticuerpos plaquetarios par los métodos de Deykin y Shulman—Sangre (Barcelona)20, 477, 1975.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Leone, G., Breda, M., Accorrà, F. et al. Platelet antibody determination by platelet factor 3 assay. La Ricerca Clin. Lab. 8, 141–147 (1978). https://doi.org/10.1007/BF02904986
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02904986