Summary
The specific IgE response that appears in subjects immunized with tetanus toxoid does not induce hypersensitivity reactions at subsequent immunizations. The type I immune response, therefore, was studied bothin vivo andin vitro, in 11 subjects who had specific IgE antibodies for tetanus toxoid. The results showed that: 1. the specific IgE antibodies are heterogeneous regarding their affinity for the mast cell and basophil receptors; 2. the specific IgG antibodies for tetanus toxoid, at serum concentrations, are not able to interfere with thein vitro specific basophil degranulation; 3. in the PEG precipitare there are aggregates of specific IgE antibodies for tetanus toxoid.In vitro, these molecular aggregates are not able to sensitize the basophil cells.
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References
Benveniste J., Ecido T., Gutierrez Millet V., Camussi G.: Detection of immediate hypersensitivity in rabbits by direct basophil degranulation—J. Allergy clin. Immunol.59, 271, 1977.
Brostoff J., Carini C., Wright D. G., Johns P.: Production of IgE complexes by allergen challenge in atopic patients and the effect of sodium chromoglycate—Lancet1, 1268, 1979.
Ceska M., Eriksson R., Varga J. M.: Radioimmunosorbent assay of allergens—J. Allergy clin. Immunol.49, 1, 1972.
Ceska M., Lundqvist U.: A new and simple radioimmunoassay method for the determination of IgE—Immunochemistry9, 1021, 1972.
Chia D., Barnett E. V., Yamagata J., Knutsen D., Restivo C., Furst D.: Quantitation and characterization of soluble immune complexes precipitated from sera by polyethylene-glycol (PEG)—Clin. exp. Immunol.37, 399, 1979.
Conroy M. C., Adkinson F., Lichtenstein L. M.: Passive sensitization of human basophils: evidence for heterogeneity of the IgE molecule—Int. Arch. Allergy60, 106, 1979.
Godfrey R., Gradidge C., Hawksley M., Elliott E.: Differences in binding affinity of human IgE for receptors in chopped human lung—Immunology35, 581, 1978.
Levine L., Wyman L., Broderick E. J., Ipsen J.: A field study in triple immunization (diphtheria, pertussis, tetanus). Estimation of 3 antibodies in infant sera from a single weal puncture using agglutination techniques—J. Pediat.57, 836, 1960.
Miadonna A., Ortolani C., Zanussi C.: Development of IgE response in humans after tetanus toxoid immunization—Boll. Ist. sieroter. milan.58, 502, 1979.
Miadonna A., Leggieri E., Tedeschi A., Zanussi C.: Clinical significance of specific IgE determination on nasal secretion—Clin. Allergy13, 155, 1983.
Nagel J., Svec D., Waters T., Fireman P.: Development of specific IgE antibodies after immunization with tetanus-diphtheria (Td) toxoids—J. Immunol.118, 334, 1977.
Norman T.:In vivo methods of study of allergy. In:Middleton E., Reed C., Ellis E. (Eds): Allergy. Principles and practice. Mosby, Saint Louis, 1978.
Sobotka A. K., Malveaux F. J., Marone G., Thomas L. L., Lichtenstein L. M.: IgE-mediated basophil phenomena: quantitation, control, inflammatory interactions—Immunol. Rev.41, 171, 1978.
Wide L., Bennich H., Johansson S. G. O.: Diagnosis of allergy by anin vitro test for allergen antibodies—Lancetii, 1105, 1967.
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Miadonna, A., Tedeschi, A., Leggieri, E. et al. Functional characterization of specific IgE antibodies for tetanus toxoid. La Ricerca Clin. Lab. 13, 235–245 (1983). https://doi.org/10.1007/BF02904837
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DOI: https://doi.org/10.1007/BF02904837