Skip to main content
Log in

Determination of plasma antithrombin III by tanned red cell hemagglutination inhibition immunoassay

  • Original Contributions
  • Published:
Ricerca in clinica e in laboratorio

Summary

Concentrations of antithrombin III have been evaluated in normal and pathological plasmas by a new immunochemical technique, the tanned red cell hemagglutination inhibition immunoassay. This method appears to be simple, sensitive, specific and correlated with the methods that analyze the biological activities of antithrombin III.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Abildgaard U., Gravem K., Godal H. C.: Assay of progressive antithrombin in plasma— Thrombos. Diathes. haemorrh. (Stuttg.)24, 224, 1970.

    CAS  Google Scholar 

  2. Collen D., De Cock F.: Emergence in plasma during activation of the coagulation or fibrinolytic systems of neoantigens associated with the complexes of thrombin or plasmin with their inhibitors—Thrombos. Res.5, 777, 1974.

    Article  CAS  Google Scholar 

  3. Collen D., De Cock F.: A tanned red cell hemagglutination inhibition immunoassay (TRCHII) for the quantitative estimation of thrombin-antithrombin III and plasmin-α1-antiplasmin complexes in human plasma—Thrombos. Res.7, 235, 1975.

    Article  CAS  Google Scholar 

  4. Gandolfo G. M., De Angelis A., Torresi M. V.: Determination of antithrombin III activities by different methods in diabetic patients—Haemostasis9, 15, 1980.

    PubMed  CAS  Google Scholar 

  5. Gandolfo G. M., Torresi M. V.: The influence of heparin on the immunochemical evaluation of antithrombin III by electroimmunoassay—Haematologica (Pavia)63, 631, 1973.

    Google Scholar 

  6. Hedner V., Nilsson I. M.: Antithrombin III in a clinical material—Thrombos. Res.3, 631, 1973.

    Article  CAS  Google Scholar 

  7. Kauffman R. H., Veltkamp J. J., Van Tilburg N. H., Van Es L. A.: Acquired antithrombin III deficiency and thrombosis in the nephrotic syndrome—Amer. J. Med.65, 607, 1978.

    Article  Google Scholar 

  8. Merskey C., Lalezari P., Johnson A. J.: A rapid, simple, sensitive method for measuring fibrinolytic split products in human serum—Proc. Soc. exp. Biol. (N.Y.)131, 871, 1969.

    CAS  Google Scholar 

  9. Miller-Andersson M., Borg H., Andersson L. O.: Purification of antithrombin III by affinity chromatography—Thrombos. Res.5, 439, 1974.

    Article  CAS  Google Scholar 

  10. O'Brien J. R.: Antithrombin III and heparin cofactor times in thrombosis and atherosclerosis. —Thrombos. Diathes. haemorrh. (Stuttg.)32, 116, 1974.

    Google Scholar 

  11. Ødegård O. R., Lie M., Abildgaard U.: Heparin cofactor activity with an amidolytic method. —Thrombos. Res.6, 287, 1975.

    Article  Google Scholar 

  12. Ødegård O. R., Lie M., Abildgaard U.: Antifactor Xa activity measured with amidolytic methods—Haemostasis5, 265, 1976.

    PubMed  Google Scholar 

  13. Sagar S., Stamatakis J. D., Thomas D. P., Kakkar V. V.: Oral contraceptives, antithrombin III activity and postoperative deep-vein thrombosis—Lanceti, 509, 1976.

    Article  Google Scholar 

  14. Sas G., Blasko G., Ganhegyi J., Jako J., Palos A.: Abnormal antithrombin III (antithrombin III ‘Budapest’) as a cause of a familial thrombophilia—Thrombos. Diathes. haemorrh. (Stuttg.)32, 105, 1974.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

This work was supported by grant no. 79.01969.04 from theConsiglio Nazionale delle Ricerche (CNR), Roma, Italy.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gandolfo, G.M., Torresi, M.V. Determination of plasma antithrombin III by tanned red cell hemagglutination inhibition immunoassay. La Ricerca Clin. Lab. 13, 229–233 (1983). https://doi.org/10.1007/BF02904836

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02904836

Key-words

Navigation