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Insulin-like growth factor-1 gene cloning and protein expression in bovine trabecular meshwork tissue and cells

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Summary

Whether cultured bovine trabecular meshwork cells and trabecular tissue ex vivo express insulin-like growth factor-I (IGF-I) messenger RNA (mRNA) and protein was investigated. Total RNA of cultured bovine trabecular meshwork cells as well as trabecular meshwork tissue freshly excised from bovine eyes was extracted, and reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect IGF-I mRNA. RT-PCR product was verified by sequencing. Immunohistochemical stain was used to detect IGF-I protein. The results showed that a single PCR amplified product was obtained, and the sequence was homologous to the known sequence. IGF-I immunostain was positive in the cytoplasm of trabecular meshwork cells. It was concluded that trabecular meshwork cells produce IGF-I and contribute to the presence of IGF-I in trabecular meshwork microenvironment as well as aqueous humor. Trabecular meshwork cells were affected by IGF-I not only through paracrine, but also autocrine action. Whether abnormal down-regulations in IGF-I production may contribute to the pathogenesis of primary open-angle glaucoma and the possibility of promoting the autocrine action of IGF-I by trabecular meshwork cells to treat the diesease is worth further investigation.

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References

  1. Quigley H A. Number of people with glaucoma worldwide. Br J Ophthalmol, 1996, 80: 389

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Grierson I, Hogg R. The proliferative and migratory activities of trabecular meshwork cell. Retinal Cell Res, 1995, 15: 33

    Google Scholar 

  3. Tripathi R C, Yang C, Tripathi B J et al. Role of receptors in the trabecular meshwork of the eye as targeted to the development of antiglaucomatherapy. Drug Dev Res, 1992, 27: 191

    Article  CAS  Google Scholar 

  4. Tripathi R C. Growth factors in the aqueous humor and their clinical significance. J Glaucoma, 1994, 3: 248

    Article  CAS  PubMed  Google Scholar 

  5. Arnold D R, Moshavedi P, Schoen T J et al. Distribution of IGF- I and-I, IGFBPs and IGFBP mRNA in ocular fluids and tissues: potential sites of synthesis of IGFBPs in aqueous and vitreous. Exp Eye Res, 1993, 56: 555

    Article  CAS  PubMed  Google Scholar 

  6. Tripathi R C, Tripathi B J, Raja S C. Prospects for growth factors therapy of cataracts. Sci Res J, 1989, 1: 131

    Google Scholar 

  7. Waldbillig R J, Schoen T, de Juan E et al. Characterization of insulin-like growth factor binding proteins in vitreous. Invest Ophthalmol Vis Sci, 1990, 31 (Suppl.): 227

    Google Scholar 

  8. Wordinger R J, Clark A F, Agarwal R et al. Cultured human trabecular meshwork cells express functional growth factor receptors. Invest Ophthalmol Vis Sci, 1998, 39: 1575

    CAS  PubMed  Google Scholar 

  9. Wirtz M K, Xu H, Rust K et al. Insulin-like growth factor binding protein-5 expression by human trabecular meshwork. Invest Ophthalmol Vis Sci, 1998, 39: 45

    CAS  PubMed  Google Scholar 

  10. Cao Y, Wei H, Da B et al. Effect of TGF-β2 on phagocytosis in cultured trabecular meshwork cells. J Tongji Med Univ, 2001, 21: 318

    Article  CAS  PubMed  Google Scholar 

  11. Wang Q, Wei H, Fan Z et al. Effect of norfloxacin and clonidine on human trabecular meshwork cellsin vitro. Graefe's Arch Clin Exp Ophthalmol, 1994, 232, 566

    Article  CAS  Google Scholar 

  12. Cao Y, Wei H, Fu M et al. Expression of TGF-β and TGF-β receptors protein in cultured bovine trabecular meshwork cells. Chin Ophthalmic Res, 2000, 18: 235

    CAS  Google Scholar 

  13. Pfaffl M, Meyer H, Sauerwein H. Quantification of insulin-like growth factor-I (IGF-I) mRNA: Development and validation of an internally standardised competitive reverse transcription-polymerase chain reaction. Exp Clin Endocrinol Diabetes, 1998, 106: 506

    Article  CAS  PubMed  Google Scholar 

  14. Fotsis T, Murphy C, Gannon F. Neucleotide sequence of the bovine insulin-like growth factor (IGF-I) and its IGF-I A precursor. Nucleic Acids Res, 1990, 18: 676

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Alvarado J, Murphy C, Juster R. Trabecular meshwork cellularity in primary open angle glaucoma and nonglaucomatous normals. Ophthalmology, 1984, 91: 564

    Article  CAS  PubMed  Google Scholar 

  16. Lutjen-Drecoll E, Shimizu T, Rohrbach M et al. Quantitative analysis of plaque material' in the inner and outer walls of Schlemm's canal in normal and glaucomatous eyes. Exp Eye Res, 1986, 42: 443

    Article  CAS  PubMed  Google Scholar 

  17. Tripathi R C, Borisuth N S C, Tripathi B J et al. Growth factors in the aqueous humor and their therapeutic implications in glaucoma and anterior segment disorders of the human eye. Drug Dev Res, 1991, 2: 1

    Google Scholar 

  18. Rechler M M, Nissley S P. Insulin-like growth factors. In: Sporn MB, Roberts AB, eds. Peptide growth factors and their receptors: handbook of experimental pharmacology. Heidelberg: Springer-Verlag, 1990, 7.1–7.2

    Google Scholar 

  19. Ullrich A, Gray A, Tam A W et al. Insulin-like growth factor- I receptor primary structure: comparison with insulin receptor structural determinants that define function specifity. EMBO J, 1986, 5: 2503

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Department of Ophthalmology, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan

    Cao Yang, Wei Houren & Da Banghong

  2. Institute of Physiology, Technical University of Munich, Freising-Weihenstephan, Germany

    Pfaffl Michael

  3. Department of Anatomy, Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan

    Li Zhongyu

Authors
  1. Cao Yang
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  2. Wei Houren
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  3. Da Banghong
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  4. Pfaffl Michael
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  5. Li Zhongyu
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Additional information

CAO Yang, male, born in 1972, M. D., Ph. D, Doctor in Charge

This project was supported by a grant from National Natural Sciences Founction of China (No. 38970758).

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Yang, C., Houren, W., Banghong, D. et al. Insulin-like growth factor-1 gene cloning and protein expression in bovine trabecular meshwork tissue and cells. Current Medical Science 22, 69–72 (2002). https://doi.org/10.1007/BF02904794

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  • DOI: https://doi.org/10.1007/BF02904794

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