Summary
To investigate whether the TGF-β1 plasmid DNA carried by lipofectamine could be introduced into cultured rabbit corneal epithelial cells, specific expression of the plasmid pMAM TGF-β1 in the cultured corneal epithelial cells was studied. Two days after 12 h of transfection of pMAMT-GF-β1 mediated by lipofectamine into the cultured corneal epithelial cells, the TGF-β1 protein expression specific for pMAMTGF-β1 in the cells was detected by means of immunohistochemical staining and the positive rate was 23.37%. The results suggested that foreign plasmid DNA could be effectively delivered into cultured rabbit corneal epithelial cells by means of lipofectamine, and this will provide a promising method of studying TGF-β1 on the mechanism of physiology and pathology concerned with corneal epithelial cells.
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HUANG Qiong, female, born in 1977, Doctor in Charge
This project was supported by a grant from the Natural Sciences Foundation of Hubei Province (N0. 97J070).
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Qiong, H., Yanhua, H., Fagang, J. et al. Experimental study of plasmid TGF-β1 DNA gene transfer with lipofectamine into rabbit corneal epithelial cellsin vitro. Current Medical Science 22, 62–65 (2002). https://doi.org/10.1007/BF02904792
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DOI: https://doi.org/10.1007/BF02904792