Summary
In order to investigate the effects of LPS on the TACE gene transcription and expression and its regulating effect on the TM-TNF secretion, in vitro studies were carried out on HL-60 cells stimulated by LPS. TACE, TNF-α mRNA levels were detected by Dot-Elisa and the distribution of membrane molecules determined by flow cytometry assay and indirect immunofluorescence. The results showed that: (1) TACE was detected in or on HL-60 cells and it is predominantly localized on cell surface and to a perinuclear compartment. (2) LPS induced a time dependent increasement of TNF-α mRNA and enhanced TNF conversion with decreasing distribution of TNF in cell surface and increasing secretion of TNF protein. Such conversion could be inhibited by TACE ODN. (3) LPS also induced time-dependently increased expression of TACE gene and activation of its function. On the other hand, TACE protein in cell lysate and on cell surface was decreased. It was suggested that TACE molecular structure might change following its mediating membrane-anchored molecular secretion.
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References
Mullberg J, Althoff K, Jostock Tet al. The importance of shedding of membrane proteins for cytokine biology. Eur Cytokine Netw, 2000, 11(1):27
Moss M L, Jin S L, Milla M Eet al. Cloning of a disintegrin metalloproteinase that processes precursor tumour-necrosis factor-alpha, Nature, 1997,385:733
Peschon J J, Slack J L, Reddy Pet al. An essential role for ectodomain shedding in mammalian development. Science, 1998,13(282):1281
Amin A R. Regulation of tumor necrosis factor-alpha and tumor necrosis factor converting enzyme in human osteoarthritis. Osteoarthritis Cartilage, 1999,7(4):392
Takashiba S, Van Dyke T, Shapira Let al. Lipopolysaccharide-Inducible and Salicylate-Sensitive Nuclear Factor on Human Necrosis Factor Alpha Promoter. Infection and Immunity, 1995, Apr 63(4):1529
Reddy P, Slack J L, Davis Ret al. Functional analysis of the domain structure of tumor necrosis factor-alpha converting enzyme. J Biol Chem, 2000, 275:14608
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LI Lingbo, female, born in 1969, M. D., Ph. D
This project was supported by a grant from National Natural Sciences Fundation of China (No. 30070722).
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Lingbo, L., Yuzhen, Y., Zhen, W. et al. Study of the effects of LPS on the TACE gene expression and its function. Current Medical Science 22, 5–8 (2002). https://doi.org/10.1007/BF02904774
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DOI: https://doi.org/10.1007/BF02904774