Abstract
Nitrate reductase level in leaves of pea plants is higher than in roots despite of the lower content of endogenous nitrate. Addition of ammonium ions to nutrient solution containing nitrate decreases nitrate reductase level in leaves estimatedin vivo while its level estimatedin vitro is increased.
Glutamine synthetase (GS) level in roots decreases during short (24 and 48 h) and long (14 d) term cultivation of seedlings in solutions containing ammonium ions. This decrease occurs in leaves only after the long term influence of ammonium ions. Level of this enzyme is higher in plants grown in the presence of nitrogen (ammonium and nitrate) as compared to those grown without the nitrogen.
Level of glutamate dehydrogenase in roots is increased after both short and long term cultivation of plants in the presence of ammonium ions.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ashley, D. A., Jackson, W. A., Volk, R. J.: Nitrate uptake and assimilation by wheat seedlings during initial exposure to nitrate. - Plant Physiol.55: 1102–1106, 1975.
Breteler, H., Hänisch Ten Cate, C. H.: Fate of nitrate during initial nitrate utilization by nitrogen-depleted dwarf bean. - Physiol. Plant.48: 292–296, 1980.
Buczek, J., Burzynski, M.: Nitrate reductase activity and yield of dry mass and protein content in cucumber seedlings supplied with nitrate and ammonium. - Acta Soc. Bot. Pol.48: 465–471, 1979.
Canvin, D. T., Atkins, C. A.: Nitrate, nitrite and ammonium assimilation by leaves: effect of light, carbon dioxide and oxygen. - Planta116: 207–224, 1974.
Ferrari, T. E., Yoder, O. C., Filner, P.: Anaerobic nitrite production by plant cells and tissues: evidence for two nitrate pools. - Plant Physiol.51: 423–431. 1973.
Givan, C. V.: Metabolic detoxication of ammonia in tissues of higher plants. - Phytochemistry18: 375–382, 1979.
Jackson, W. A., Fletcher, D., Hageman, R. H.: Nitrate uptake by dark-grown corn seedlings (some characteristics of apparent induction). - Plant Physiol.37: 120–127, 1973.
Joy, K. W.: Glutamate dehydrogenase changes inLemna not due to enzyme induction. - Plant Physiol.47: 445–446, 1971.
Keys, A. J., Bird, I. F., Cornelius, M. J., Lea, P. J., Wallsgrove, R. M., Miflin, B. J.: Photorespiratory nitrogen cycle. - Nature275: 741–743, 1978.
Lastůvka, L., Minár, V.: [Water cultures of higher plants.] In Czech. - Folia Fac. Sci. Nat. Univ. brun. VIII: 1–83, 1967.
Lea, P. J., Miflin, B. J.: An alternative route for nitrogen assimilation in higher plants. - Nature251: 614–616, 1974.
Miflin, B. J., Lea, P. J.: The pathway of nitrogen assimilation in plants. - Phytochemistry15: 873–886, 1976.
Oaks, A., Stulen, I., Boesel, I. L.: Influence of amino acids and ammonium on nitrate reduction in corn seedlings. - Can. J. Bot.57: 1824–1829, 1979.
Pate, J. S.: Uptake, assimilation and transport of nitrogen compounds by plants. - Soil Biol. Biochem.5: 109–119, 1973.
Paul, J. L., Carlson, R. M.: Nitrate determination in plant extracts by nitrate electrode. - J. agr. Food Chem.16: 766–768, 1968.
Radin, J. W.: Contribution of the root system to nitrate assimilation in whole cotton plants. - Aust. J. Plant Physiol.4: 811–819, 1977.
Rhodes, D., Sims, A. P., Stewart, G. R.: Glutamine synthetase and the control of nitrogen assimilation inLemna minor L. - In:Hewitt, E. J., Cutting, C. V. (ed.): Nitrogen Assimilation of Plants. Pp. 501–520. Acad. Press, London 1979.
Sahulka, J., Gaudinová, A., Hadacová, V.: Regulation of glutamate dehydrogenase and nitrate reductase levels in excised pea roots by exogenously supplied sugars. - Z. Pflanzenphysiol.75: 392–404, 1975.
Shaner, D. L., Boyer, J. S.: Nitrate reductase activity in maize (Zea mays L.) leaves. I. Regulation by nitrate flux. - Plant Physiol.58: 499–504, 1976.
Shapiro, B. M., Stadtman, E. R.: Glutamine synthetase (Escherichia coli) biosynthetic assay, Pi measurement, transferase assay, γ-glutamyl hydroxamate measured. - In:Tabor, H., Tabor, C. N. (ed.): Methods in Enzymology. Vol. 17 A. Pp. 910–922. Acad. Press, London-New York 1970.
Skrdleta, V., Gaudinová, A., Nemcová, M.: Relationships between nitrate level, nitrate reductase activity and anaerobic nitrite production inPisum sativum leaf tissue. - Biol. Plant.21: 307–310, 1979.
Stewart, G. R., Rhodes, D.: Control of enzyme levels in regulation of nitrogen assimilation. - In:Smith, H. (ed.): Regulation of Enzyme Synthesis and Activity in Higher Plants. Annu. Proc. Phytochem. Soc. No. 14. Pp. 1–22, Acad. Press, London-New York-San Francisco 1977.
Wallace, W., Pate, J. S.: Nitrate reductase in the field pea (Pisum arvense L.). - Ann. Bot.29: 655–617, 1965.
Wray, J. Y., Filner, P.: Structural and functional relationships of enzyme activities induced by nitrate in barley. - Biochem. J.119: 715–725, 1970.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gaudinova, A. The effect of NO3- and NH4 + ions on enzymes involved in nitrogen assimilation inPisum sativum L. Biol Plant 25, 440–448 (1983). https://doi.org/10.1007/BF02903144
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02903144