Abstract
Callogenesis was induced in mature embryos. The efficiency of induction and the growth of calli were dependent on 2,4-D concentration. No regeneration of buds or shoots was observed in 720 calli studied, but most calli showed intensive root proliferation on the regeneration medium. Most of the root meristem cells maintained diploid chromosome number. Only a low proportion (about 3.5%) of tetraploid cells was found. No other chromosomal changes were observed. Chromosomal variability does not contribute to the inability of calli derived from mature barley embryos to form buds and shoots.
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Abbreviations
- IAA:
-
indolyl-3-acetic acid
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- BAP:
-
6-benzyl-aminopurine
References
Cheng, T. Y., Smith, H. H.: Organogenesis from callua cultures ofHordeum vulgare. - Planta123: 307–310, 1975.
Chin, J. C., Scott, K. J.: The isolation of a high-rooting cereal callus line by recurrent selection with 2,4-D. -Z. Pflanzenphysiol.85: 117–124, 1977.
Dale, P. J., Deambrogio, E.: A comparison of callus induction and plant regeneration from different explants ofHordeum vulgare. - Z. Pflanzenphysiol.94: 65 - 77, 1979.
Gamborg, O. L., Eveleigh, D. E.: Culture methods and detection of gluconates in suspension cultures of wheat and barley. - Can. J. Biochem.46: 417–421, 1968.
Landová, B., Ondřej, M.: Short term cultivation of isolated barley roots and their mitotic activity.- Biol. Plant.21: 113–118, 1979.
Novák, F. J.: Chromosomal instabilities in callus tissue from haploid barley (Hordeum vulgare). - Biol. Plant.22: 303–305, 1980.
Novák, F. J.:In vitro plant regeneration from callus tissue of cereals (barley, wheat and triticale). - In: Proc. 7th World Cereal and Bread Congr. Elsevier Sci. Publ. Co., Amsterdam 1982.
Orton, T. J.: A quantitative analysis of growth and regeneration from tissue cultures ofHordeum vulgare., Hordeum jubatum and their interspecific hybrid. - Environ. exp. Bot.19: 319–335, 1979.
Orton, T. J.: Chromosomal variability in tissue cultures and regenerated plants ofHordeum. - Theor. appl. Genet.56: 101 - 112, 1980.
Orton, T. J., Steidl, R. P.: Cytogenetic analysis of plants regenerated from colchicine-treated callus cultures of an interspecificHordeum hybrid. - Theor. appl. Genet.57: 89 - 90, 1980.
Saalbach, G., Koblitz, H.: Karyological instabilities in callus cultures from haploid barley plants. - Biochem. Physiol. Pflanzen171: 469–473, 1977.
Scheunert, E. U., Shamina, Z. B., Koblitz, H.: Studies on barley callus culturesin vitro. I. Establishment, maintenance and growth of two different tissue strains. - Plant Sci. Lett.10: 313–318, 1977.
Scheunert, E. U., Shamina, Z. B., Koblitz, H.: Karyological features of barley callus tissues culturedin vitro. - Biol. Plant.20: 305–308, 1978.
Sozinov, A., Lukjanuk, S., Ignatova, S.: Anther cultivation and induction of haploid plants inTriticale. - Z. Pflanzenzücht.86: 272 - 285, 1981.
Yamada, Y.: Tissue culture studies in cereals. - In:Reinert, J., Bajaj, Y. P. S. (ed.): Applied and Fundamental Aspects of Plant Cell, Tissue and Organ Culture. Pp. 144–159. Springer-Verlag, Berlin 1977.
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Vostřáková-Němcová, B., Rakouský, S. & Ondřej, M. Chromosomal characteristics of barley root meristems differentiated from calli. Biol Plant 25, 288–292 (1983). https://doi.org/10.1007/BF02902876
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DOI: https://doi.org/10.1007/BF02902876