Abstract
Nitrate concentration required for maximal extractable level of nitrate reductase (NR) inWolffia varies with the conditions prior to the nitrate treatment. Maximal enzyme activity is obtained at 2 mM nitrate concentration with asparagine grown plants and at 15 mM with nitrate grown. Both the level of enzyme activity and nitrate uptake by the tissue are increased by irradiation. The radiant energy induced increase in enzyme activity is not due to photosynthetic activity alone. An effect of radiant energy at the membrane level is sug gested. The extracted enzyme, which is labile, is protected and activated by NADH at 0 °C.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aslam, M., Oaks, A.: Effect of glucose on the induction of nitrate reductase in corn roots. - Plant Physiol.56: 634–639, 1975.
Aslam, M., Oaks, A., Huffaker, R. C.: Effect of glucose on the induction nitrate reductase and on distribution of nitrate in etiolated barley leaves. - Plant Physiol.58: 588 - 591, 1976.
Bakshi, I. Singh, Abad Farooqi, A. H., Maheshwari, S. C.: Circadian rhythm in nitrate reductase activity in the duckweedWolffia microscopica Griff. - Z. Pflanzenphysiol.90: 165 to169, 1978.
Bakshi, I. Singh, Abad Farooqi, A. H., Maheshwari, S. C.: Control of circadian rhythm in nitrate reductase activity inWolffia microscopica Griff. - Plant Cell Physiol.20: 957 to 963, 1979.
Barea, J. L., Maldonado, J. M., Cardenas, J.: Further characterization of nitrate and nitrite reductases fromChlamydomonas reinhardii. - Physiol. Plant.36: 325 - 332, 1976.
Ferrari, T. E., Yoder, O. C., Filner, P.: Anaerobic nitrite production by plant cell and tissues — evidence for two nitrate pools. - Plant Physiol.51: 423 - 431, 1973.
Hewitt, E. J.: Assimilatory nitrate and nitrite reduction. - Annu. Rev. Plant Physiol.26: 73 to 100, 1975.
Jaworski, E. G.: Nitrate reductase assay in intact plant tissues. - Biochem. biophys. Res. Commun.43: 1274–1279, 1971.
Jones, R. W., Sheard, R. W.: Nitrate reductase activity: Phytochrome mediation of induction in etiolated peas. -Nature-new Biol.238: 221 - 222, 1972.
Jones, R. W., Sheard, R. W.: Blue light enhanced nitrate reductase in etiolated pea terminal buds. - Can. J. Bot.52: 1433–1435, 1974.
Kadam, S. S., Johri, R. P., Ramarao, C. S., Srinivasan: Stability of nitrate reductase and source of its reductant inSorghum seedlings. - Phytochemistry19: 2095 - 2097, 1980.
Kadam, S. S., Sawhney, S. K., Naik, M. S.: Effect of NADH on the activities of nitrate reductase and its inactivating enzyme. - Indian J. Biochem. Biophys.12: 81 - 85, 1975.
Klepper, L., Flesher, D., Hageman, R. H.: Generation of reduced nicotinamide adenine dinucleotide for nitrate reduction in green leaves. - Plant Physiol.48: 580–590, 1971.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with Folin-phenol reagent. - J. Biol. Chem.193: 265–275, 1951.
Maldonado, J. M., Herrera, J., Paneque, A., Losada, M.: Reversible inactivation by NADH and ADP ofChlorella fusca nitrate reductase. - Biochem. biophys. Res. Commun.51: 27 to 33, 1973.
Palacián, E., De la Rosa, F., Carillom, F., Gómez-Moreno, C.: Nitrate reductase fromSpinacea oleracea. Reversible inactivation by NAD(P)H and by thiols. - Arch. Biochem. Biophys.161: 441–447, 1974.
Radin, J. W., Parker, L. L., Sell, C. R.: Partitioning of sugar between growth and nitrate reduction in corn roots. - Plant Physiol.62: 550–553, 1978.
Rai, A. K., Kashyap, A. K., Gupta, S. L.: ATP-dependent uptake of nitrate inNostoc muscorum and inhibition by ammonium ions. - Bioehem. biophys. Acta674: 78–86, 1981.
Sahulka, J., Lisá, L.: Effect of some disaccharides, hexoses and pentoses on nitrate reductase, glutamme synthetase, glutamate dehydrogenase in excised pea roots. - Physiol. Plant.50: 32–36, 1980.
Sluiters-Scholten, C. M. Th.: Photosynthesis and the induction of nitrate reductase and nitrite reductase in bean leaves. -Planta123: 175–184, 1975.
Solomonson, L. P.: Regulation of nitrate reductase activity by NADH and cyanide. - Biochim. biophys. Acta334: 297–308, 1974.
Stewart, G. R.: The regulation of nitrite reductase level inLemna minor L. - J. exp. Bot.23: 171–183, 1972.
Swader, J. A., Stocking, C. R., Chin Ho Lin: Light stimulated absorption of nitrate byWolffia arrhiza. -Physiol. Plant.34: 335–341, 1975.
Tischler, C. R., Purvis, A. C., Jordon, W. R.: Factors involved inin vitro stabilization of nitrate reductase from cotton (Gossypiumhirsutum L.) cotyledons. - Plant Physiol.61: 714 to 717, 1978.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bakshi, I.S. Effects of asparagine, Irradiation and NADH on the nitrate reductase inWolffia microscopica . Biol Plant 25, 259–265 (1983). https://doi.org/10.1007/BF02902872
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02902872