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Callus formation and plant regeneration from protonemal protoplasts of the mossAnoectangium thomsonii Mitt.

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Biologia Plantarum

Abstract

The protoplasts of the mossAnoectangium thomsonii, isolated from protonemal cells underwent divisions in 48–72 hours on modified MS medium enriched with growth regulators, 2,4-D and kinetin, 10 % sucrose and coconut water (5 %). Subculture of protoplast derived cells in a medium of relatively low osmotic potential (5 % sucrose) produced dark green calli which could be maintained completely undifferentiated.

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Author notes

  1. Praveen K. Saxena

    Present address: Department of Biology, University of Saskatchewan, S7N OWO, Saskatoon, Canada

Authors and Affiliations

  1. Department of Botany, University of Delhi, 11007, Delhi

    Praveen K. Saxena

  2. Bio-Organic Division, Bhabha Atomic Research Centre, Bombay, India

    R. Gill

Authors
  1. Praveen K. Saxena
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  2. R. Gill
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Saxena, P.K., Gill, R. Callus formation and plant regeneration from protonemal protoplasts of the mossAnoectangium thomsonii Mitt. . Biol Plant 28, 310–312 (1986). https://doi.org/10.1007/BF02902301

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  • DOI: https://doi.org/10.1007/BF02902301

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