Summary
109 biopsy samples from 35 HBAg serologically positive and 74 negative patients were examined by IFL for the presence of the surface and core antigenic determinants associated with the Dane particle. In no serologically negative case was specific IFL detected. Different patterns were observed in serologically positive patients: negative in acute hepatitis, strongly positive cytoplasmic HBs fluorescence in chronic HBAg carriers with normal liver, and discrete HBsAg parenchymal and mesenchymal staining and variable HBcAg staining in chronic liver disease, with HBsAg appearing more frequently in inactive and HBcAg in active disease. These results are compared with recent reports in this field and the clinical significance of the intrahepatic localization of HBAg is discussed.
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References
Alberti A., Realdi G., Tremolada F., Cadrobbi P.: HBAg on Liver Cell in Viral Hepatitis — Lancet1, 346, 1975.
Almeida J. D., Rubenstein D., Stott E. J.: New Antigen-Antibody System in Australia Antigen-Positive Hepatitis — Lancet2, 1225, 1971.
Blumberg B. S., Alter H. J., Visnich S. A.: A New Antigen in Leukemia Sera — J. Amer. med. Ass.191, 541, 1965.
Brzosko W. J., Madalinsky K., Krawczynsky K., Nowoslawsky A.: Duality of Hepatitis B Antigen and Its Antibody. I. Immunofluorescence Studies — J. infect. Dis.127, 424, 1973.
Caramia F., de Bac C., Ricci G.: Flectron Microscopy Studies of Severe Virus Hepatitis. Cytoplasmic Inclusions, Virus-Like Particles, Australia Antigen — Amer. J. Dis. Child.123, 309, 1972.
Ceppellini R., Bedarida G., Carbonara A. O., Trinchieri G., Filippi G.: High Frequency of Family Clustering of Au Antigen in Some Italian Populations — In: Proceedings of the Convegno Farmitalia sull’Antigene Australia. Minerva Medica, Torino, 1970.
Cerat G., Richer G., Viallet A., Coté J., Robert J., Turgeon F.: Detection of Australia Antigen in Liver Biopsies by Immunofluorescence — Canad. med. Ass. J.108, 981, 1973.
Coyne V. E., Millman I., Cerda J., Gerstey B. J. S., London T., Sutnick A., Blumberg B. S.: The Localization of Australia Antigen by Immunofluorescence — J. exp. Med.131, 307, 1970.
Dane D. S., Cameron C. H., Briggs M.: Virus-Like Particles in Serum of Patients with Australia Antigen-Associated Hepatitis — Lancet1, 695, 1970.
de Groote J., Desmet V. J., Gedick P., Korb G., Popper H., Poulsen H., Schmid M., Thaler H., Uehlinger E., Wepler W.: A Classification of Chronic Hepatitis — Lancet2, 626, 1968.
Dudley F. J., Fox R. A., Sherlock S.: Cellular Immunity and Hepatitis-Associated Australia Antigen Liver Disease — Lancet1, 723, 1972.
Edgington T. S., Ritt D. J.: Intrahepatic Expression of Serum Hepatitis Virus-Associated Antigens — J. exp. Med.134, 871, 1971.
Gerber M. A., Hadziyannis S. J., Vissoulis C., Schaffner F., Paronetto F., Popper H.: Hepatitis B Antigen: Nature and Distribution of Cytoplasmic Antigen in Hepatocytes of Carriers — Proc. Soc. exp. Biol. (N.Y.)145, 863, 1974.
Gerber M. A., Paronetto F.: Hepatitis B Antigen in Human Tissues — In:Schaffner F., Sherlock S., Leevy C. (Eds): The Liver and Its Diseases. Intercontinental Medical Book Corporation, New York, 1973; p. 54.
Gudat F., Bianchi L., Sonnabend W., Thiel G., Aenishaenslin W., Stalder G. A.: Pattern of Core and Surface Expression in Liver Tissue Reflects State of Specific Immune Response in Hepatitis B — Lab. Invest.32, 1, 1975.
Hadziyannis S. J.: Immunofluorescence Study of the Core Antigen in the Liver — Digestion8, 437, 1973.
Hadziyannis S. J., Vissoulis C., Moussouros A., Afroudakis A.: Cytoplasmic Localization of Australia Antigen in the Liver — Lancet1, 976, 1972.
Hoofnagle J. H., Gerety R. J., Barker L. F.: Antibody to Hepatitis B Virus Core in Man — Lancet2, 869, 1973.
Huang S. N., Groh V., Beaudoin J. G., Dauphinee W. D., Guttmann R. D., Morehouse D. D., Aronoff A., Gault H.: A Study of the Relationship of Virus-Like Particles and Australia Antigen — Hum. Path.5, 209, 1974.
Krawczynsky K., Mazarewicz T., Brzosko W. J., Nowoslawsky A.: Cellular Localization of Hepatitis-Associated Antigen in Liver of Patients with Different Forms of Hepatitis — J. infect. Dis.126, 372, 1972.
Millman I., Zavatone V., Gerstley B. J. S., Blumberg B. S.: Australia Antigen Detected in the Nuclei of Liver Cells of Patients with Viral Hepatitis by the Fluorescent Antibody Technique — Nature (Lond.)222, 181, 1969.
Muller R., Maess J., Deicher H.: Fluoreszenzserologischer Nachweis von Australia Antigen in der Leber — Dtsch. med. Wschr.98, 93, 1973.
Nelson J. M., Barker L. F., Danovitch S. H.: Intranuclear Aggregates in the Liver of a Patient with Serum Hepatitis — Lancet2, 773, 1970.
Nowoslawsky A.: Quoted byCerat G. et al. 7 — Canad. med. Ass. J.108, 981, 1973.
Petts V., Roitt I. M.: Peroxidase Conjugates for Demonstration of Tissue Antibodies. Evaluation of the Technique — Clin. exp. Immunol.9, 407, 1971.
Popper H.: Communication to the Simposio Internazionale sull’epatite cronica — Montecatini, April 10–12, 1975.
Stein O., Fainaru M., Stein Y.: Visualization of Virus-Like Particles in Endoplasmatic Reticulum of Hepatocytes of Australia Antigen Carriers — Lab. Invest.26, 262, 1972.
Turbessi G., de Bac C., Caramia F., Ricci G.: I portatori di antigene dell’epatite B. Studi con immunofluorescenza sulla localizzazione epatica dell’antigene — Recenti Progr. Med.56, 212, 1974.
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Rizzetto, M., Crivelli, O., Verme, G. et al. Intrahepatic localization of the surface (HBsAg) and core (HBcAg) antigenic determinants associated with hepatitis B virus in biopsy samples from patients with liver disease. La Ricerca in Clin. Lab. 6, 41–54 (1976). https://doi.org/10.1007/BF02901488
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DOI: https://doi.org/10.1007/BF02901488