Abstract
Recombinant “all-fish” growth hormone gene (GH) was microinjected into the fertilized eggs of carp. A comparison between the growth traits of transgenics and non-transgenics was carried out, and the transgenic individuals with significant “fast-growing” effect were successfully gained. A comparison on the reproductivities was also given out between the transgenics and their non-transgenic siblings, and showed that the reproductive capacity of transgenics was substantially equivalent to those of the non-transgenics. On the other hand, the genetic separation and the characteristic distribution of the F1 generation were genetically analyzed, which gave solid evidence for the hypothesis that 2–3 chromosomes are integrated with transgene. In addition, the distinct biological effects for multisite-integrated transgenes were further discussed. The present study opens a door for the breeding of “fast-growing” transgenic fish.
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Wang, Y., Hu, W., Wu, G. et al. Genetic analysis of “all-fish” growth hormone gene trans ferred carp (Cyprinus carpio L.) and its F1 generation. Chin.Sci.Bull. 46, a1–a4 (2001). https://doi.org/10.1007/BF02900596
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DOI: https://doi.org/10.1007/BF02900596