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Subcellular localization of the stripe disease-specific protein encoded by rice stripe virus (RSV) in its vector, the small brown planthopper,Laodelphax striatellus

Abstract

The stripe disease-specific protein (SP) encoded by the rice stripe virus (RSV) was successfully used as a localization signal of the virus in its vector, the small brown lanthopper,Laodelphax striatellus Fallen. Immunogold particles in large numbers were detected in various parts of the viruliferous females: the ovum, surface of chorion, the midgut lumen, and the columnar cells. Whereas there was none of these particles in the non-viruliferous females and males, and testis of viruliferous males. Endosymbionts (mycetocytes) were abundant, harboring ovaries of both viruliferous and non-viruliferous females, but none in the testis of males. The results provided us with the direct proof that RSV is a ciruculative and propagative plant virus and it was transovarially transmitted alongside with endosymbionts of its vector. Therefore, we deem it a nice lead for future studies on the mechanism of RSV transmission and functioning of its viral proteins.

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References

  1. Okuyama, S., Yora, K., Asuyama, H., Multiplication of the rice stripe virus in its vector,Laodelphax striatellus Fallen, Ann. Phytopathol. Soc. Jpn., 1968, 34: 255.

    Google Scholar 

  2. Gingery, R. E., The rice stripe virus group, The Plant Virus, Vol 4 (ed. Milne, R. G.), New York: Plenum Press, 1988, 297–329.

    Google Scholar 

  3. LinQiying, Xie Lianhui, Zhou Zhongju et al., Study on rice stripe disease I. Distribution and losses of the disease, J. Fujian Agricultural College, 1990, 19: 421.

    Google Scholar 

  4. Ramirez, B. C., Haenni, A. L., Molecular biology of tenuiviruses, a remarkable group of plant viruses, J. Gen Virol., 1994, 75: 467.

    Article  Google Scholar 

  5. Toriyama, S., Takahashi, M., Sano, Y. et al., Nucleotide sequence of RNA1, the largest genomic segment of rice stripe virus, the prototype of tenuiviruses, J. Gen Virol., 1994, 75: 3569.

    Article  Google Scholar 

  6. Takahashi, M., Toriyama, S., Hamamatsu, C. et al., Nucleotide sequence and possible ambisense coding strategy of rice stripe virus RNA segment 2, J. Gen Virol., 1993, 74: 769.

    Article  Google Scholar 

  7. Qu, Z. C., Liang, D. L., Harper, G. et al., Comparison of sequences of RNAs 3 and 4 of rice stripe virus from China with those of Japanese isolates, Virus Genes, 1997, 15: 99.

    Article  Google Scholar 

  8. Kakutani, T., Hayano, Y., Hayashi, T. et al., Ambisense segment 3 of rice stripe virus: the first instance of a virus containing two ambisense segments, J. Gen Virol., 1991, 72: 465.

    Article  Google Scholar 

  9. Zhu, Y., Hayakawa, T., Toriyama, S. et al., Complete nucleotide sequence of RNA3 of rice stripe virus: an ambisense coding strategy, J. Gen Virol., 1991, 72: 763.

    Article  Google Scholar 

  10. Qu, Z. C., Shen, D. L., Xu, Y. N. et al., Western blotting of RSV gene products in rice and insect, Chinese Journal of Genetics, 1999, 26: 331.

    Google Scholar 

  11. Gray, S. M., Banerjee, N., Mechanisms of arthropod transmission of plant and animal viruses, Microbiol. Mol. Biol. Rev., 1999, 63: 128.

    Google Scholar 

  12. Li Ruduo, Wang Jinqi, Su Deming, Ovary Development in Insects and Forecasting of Pests, Shanghai: Fudan University Press, 1987, 40–44.

    Google Scholar 

  13. Li Shaoling, Zhang Xianghong, Hao Jing et al., Improved immunogold labeling technique, Journal of Shangdong Medical University, 2000, 38: 12.

    Google Scholar 

  14. Beard, C. B., O’Neill, S. L., Tesh, R. B. et al., Modification of arthropod vector competence via symbiotic bacteria, Parasitology Today, 1993, 9: 179.

    Article  Google Scholar 

  15. Toriyama, S., Rice stripe virus: prototype of a new group of viruses that replicate in plant and insects, Microbiological Sciences, 1986, 3: 347.

    Google Scholar 

Download references

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Correspondence to Deming Su.

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Wu, A., Zhao, Y., Qu, Z. et al. Subcellular localization of the stripe disease-specific protein encoded by rice stripe virus (RSV) in its vector, the small brown planthopper,Laodelphax striatellus . Chin.Sci.Bull. 46, 1819–1822 (2001). https://doi.org/10.1007/BF02900559

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  • DOI: https://doi.org/10.1007/BF02900559

Keywords

  • Laodelphax striatellus
  • rice stripe virus
  • midgut
  • ovary
  • immunogold labeling
  • immunoelectron microscopy