Abstract
A sensitive radioimmunoassay for gonadotropin releasing hormone has been developed. The assay has been validated for its specificity by testing various analogues of gonadotropin releasing hormone. Analysis of plasma samples during the menstrual cycle of 4 female bonnet monkeys showed a significant increase in the immunoreactive gonadotropin releasing hormone levels during preovulatory period of the menstrual cycle.
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Abbreviations
- GnRH:
-
Gonadotropin releasing hormone
- CG:
-
chorionic gonadotropin
- RIA:
-
radioimmunoasay
- GPBS:
-
Gelatin-phosphate buffered saline 0.01 M, pH 7.4
References
Ashitaka, Y., Nishimura. R., Takemore, M. and Tojo, S. (1980) inChorionic gonadotropin (ed. Sheldon J. Segal) (New York: Plenum) p. 147.
Crowley, W. F. Jr., Filicori, M., Spratt, D. I. and Santoro, N. F. (1985)Recent Prog. Hrom. Res.,41, 473.
Elkind-Hirsch. K., Ravniker. V., Schiff. I., Tulchinsky, D. and Ryan. K. J. (1982)J. Clin. Endocrinol. Metab.,54, 602.
Gautron, J. P., Pattou, E. and Kordon, C. (1981)Mol. Cell. Endocrinol.,24, 1.
Hichens, M., Gale, P. H. and Schawan, H. (1974) inMethods in Radioimmunoassay (eds M. B. Jaffe and R. H. Behrman) (New York: Academic Press Inc.) p. 45.
Khodr, G. S. and Siler-Khodr, T. M. (1978)Fertil. Steril.,30, 301.
Khodr, G. S. and Siler-Khodr, T. M. (1980)Science,207, 315.
Koch, Y., Wilchek, M., Fridkin, M., Chobsieng, P., Zor, U. and Lindner, H. R. (1973)Biochem. Biophys. Res. Commun.,55, 616.
Nowak, R. A., Wiseman, B. S. and Bahr, J. M. (1984)Biol. Reprod.,31, 67.
Prahlada, S., Mukku, V. R., Rao, A. J. and Moudgal, N. R. (1975)Contraception,12, 137.
Rao, A. J., Mathialagan, N., Kotagi, S. G. and Moudgal, N. R. (1984a)J. Biosci.,6, 97.
Rao, A. J. and Moudgal, N. R. (1984b)IRCSMed. Sci.,12, 1105.
Rao, A. J., Kotagi, S. G. and Moudgal, N. R. (1984c)J. Reprod. Fertil.,70, 449.
Seeberg, P. H. and Adelman, J. P. (1984)Nature (London),311, 666.
Siler-Khodr, T. M., Khodr, G. S., Valenzeula, G. and Rhode, J. (1986)Biol. Reprod.,34, 245.
Tan, L. and Rousseau, P. (1982)Biochem. Biophys. Res. Commun.,109, 1061.
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Mathialagan, N., Rao, A.J. Plasma levels of gonadotropin releasing hormone during menstrual cycle ofMacaca radiata . J. Biosci. 10, 423–428 (1986). https://doi.org/10.1007/BF02900502
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DOI: https://doi.org/10.1007/BF02900502