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Correlation between human platelet cytoplasmic membrane outer leaflet fluidity, Na+/H+ exchanger activity and aging

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Abstract

A cross-sectional survey was undertaken to study the aging effect on platelet cytoplasmic membrane outer leaflet fluidity and Na+/H+ antiporter activity, and to investigate the relative influence of membrane order and age on the Na+/H+ exchanger. The study population consisted of 19 healthy subjects (age range 22–83 years, mean age 48.5 ± 16.5 years; 12 females and 7 males). Washed platelets were used as neuronal models and as eventually contributing to brain pathology by aberrant secretion of their granule content. A significant positive correlation (r = 0.48,p < 0.05) was found between age and the membrane structural order, according to a second-degree equation. Sewall-Wright path coefficient analysis revealed that the age influence on Na+/H+ exchanger activity was unimportant (0.18), but those of cytoplasmic membrane outer leaflet fluidity and mean platelet volume were strong and positive (0.51 and 0.54, respectively). Women presented a marked standard deviation in the countertransporter results. In conclusion, decreased membrane outer leaflet fluidity and unchanged Na+/H+ antiporter activity may contribute to the process of normal brain aging.

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Authors and Affiliations

  1. Center for Neuroscience of Coimbra, Laboratory of Biochemistry, School of Medicine, University of Coimbra, P-3000, Coimbra, Portugal

    Carlos F. M. Marinho & Catarina R. Oliveira

  2. Neurologic Clinic, University of Coimbra Hospital, P-3000, Coimbra, Portugal

    Catarina R. Oliveira

  3. Department of Biochemistry, School of Medicine, University of Oporto, P-4200, Oporto, Portugal

    Carlos F. M. Marinho & José Pinto-de-Barros

  4. Department of Hygiene and Epidemiology, School of Medicine, University of Oporto, P-4200, Oporto, Portugal

    Joaquim Costa-Maia

Authors
  1. Carlos F. M. Marinho
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  2. Catarina R. Oliveira
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  3. José Pinto-de-Barros
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  4. Joaquim Costa-Maia
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Marinho, C.F.M., Oliveira, C.R., Pinto-de-Barros, J. et al. Correlation between human platelet cytoplasmic membrane outer leaflet fluidity, Na+/H+ exchanger activity and aging. Eur Arch Psychiatry Clin Nuerosci 247, 275–277 (1997). https://doi.org/10.1007/BF02900306

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  • DOI: https://doi.org/10.1007/BF02900306

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