Abstract
The content of sterols and lipids was compared in the cells ofSaccharomyces cerevisiae cultivated in sporulation and the sterol-induction nitrogen-limited media. After 24 h the measured values in the two cultivations did not significantly differ. However, after subsequent 24 h, further formation of lipid globules and a corresponding increase of lipid and sterol content was detected only in the sterol-induction medium. To demonstrate the similarity of physiological state during the first day of the two cultivations, the combined cultivations were performed. Maximum sporulation, suggesting maximum similarity, of the two processes was achieved when the cells were grown in the sterol-induction medium for 15 h and then transferred to a sporulation medium.
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Abbreviations
- IMM:
-
sterol-induction molasses medium
- RSM:
-
rich sporulation medium
- PSM:
-
poor sporulation medium
- OJ:
-
Olson-Johnson medium
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Běhalov’a, B., Hoza, P., Bl’ahov’a, M. et al. Effect of nitrogen limitation and sporulation on sterol and lipid formation inSaccharomyces cerevisiae . Folia Microbiol 37, 442–449 (1992). https://doi.org/10.1007/BF02899903
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DOI: https://doi.org/10.1007/BF02899903