Summary
The nephritogenic antigen that induces antiglomerular basement membrane antibody-induced glomerulonephritis (anti-GBM nephritis) in rats was isolated from collagenase-solubilized bovine renal basement membranes. Purification was achieved using antibody-coupled affinity columns which were originally used for the purification of trypsin-solubilized nephritogenic antigen (Sado et al. 1984a). The nephritogenic antigen was a heteropolymer composed of P2 (Mr 28 kDa) and P3 (Mr 30 kDa) polypeptides as monomers and their dimers in sodium-dodecyl-sulfate (SDS) polyacryl amide gel electrophoresis. The P3 polypeptide was considered to be the nephritogenic epitope, since a fraction composed of the P2 polypeptide alone was not nephritogenic.
The properties of the nephritogenic epitope were the same as those of the Goodpasture epitope (M2*), which is a noncollagenous domain of the α3 chain of type IV collagen (Butkowski et al. 1985; Saus et al. 1988), indicating that the nephritogenic antigen is the same as the Goodpasture antigen.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Butkowski RJ, Wieslander J, Wisdom BJ, Barr JF, Noelken ME, Hudson BG (1985) Properties of the globular domain of type IV collagen and its relationship to the Goodpasture antigen. J Biol Chem 260:3739–3747
Butkowski RJ, Langeveld JPM, Wieslander J, Hamilton J, Hudson BG (1987) Localization of the Goodpasture epitope to a novel chain of basement membrane collagen. J Biol Chem 262:7874–7877
Bygren P, Wieslander J, Heinegärd D (1987) Glomerulonephritis induced in sheep by immunization with human glomerular basement membrane. Kidney Int 31:25–31
Kingsbury FB, Clark CE, Williams G, Post AL (1926) The rapid determination of albumin in urine. J Lab Clin Med 11:981–989
Kleppel MM, Michael AF, Fish AJ (1986) Antibody specificity of human glomerular basement membrane type IV collagen NC1 subunits. Species variation in subunit composition. J Biol Chem 261:16547–16552
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
Langeveld JPM, Wieslander J, Timoneda J, McKinney P, Butkowski RJ, Wisdom BJ, Jr, Hudson BG (1988) Structural heterogeneity of the noncollagenous domain of basement membrane collagen. J Biol Chem 263:10481–10488
Naito I, Sado Y (1989) Early changes of rat experimental autoimmune glomerulonephritis induced with the nephritogenic antigen from bovine renal basement membranes. J Clin Lab Immunol 28:187–193
Sado Y, Okigaki T, Seno S (1983) Rat glomerulonephritis induced by soluble antigens from bovine glomerular basement membrane. Proc Jpn Acad 59B: 24–27
Sado Y, Watanabe K, Okigaki T, Takamiya H, Seno S (1984a) Isolation and characterization of nephritogenic antigen from bovine glomerular basement membrane. Biochim Biophys Acta 798:96–102
Sado Y, Okigaki T, Takamiya H, Seno S (1984 b) Experimental autoimmune glomerulonephritis with pulmonary hemorrhage in rats. The dose-effect relationship of the nephritogenic antigen from bovine glomerular basement membrane. J Clin Lab Immunol 15:199–204
Sado Y, Naito I, Akita M, Okigaki T (1986) Strain specific responses of inbred rats on the severity of experimental autoimmune glomerulonephritis. J Clin Lab Immunol 19:193–199
Sado Y, Naito I, Okigaki T (1989) Transfer of anti-glomerular basement membrane antibody-induced glomerulonephritis in inbred rats with isologous antibodies from the urine of nephritic rats. J Pathol 158:325–332
Saus J, Wieslander J, Langeveld JPM, Quinones S, Hudson BG (1988) Identification of the Goodpasture antigen as the α3(IV) chain of collagen IV. J Biol Chem 263:13374–13380
Weber S, Dölz R, Timpl R, Fessier JH, Engel J (1988) Reductive cleavage and reformation of the interchain and intrachain disulfide bonds in the globular hexameric domain NC1 involved in network assembly of basement membrane collagen (type IV). Eur J Biochem 175:229–236
Wick G, Von der Mark H, Dietrich H, Timpl R (1986) Globular domain of basement membrane collagen induces autoimmune pulmonary lesions in mice resembling human Goodpasture disease. Lab Invest 55:308–317
Wieslander J, Barr JF, Butkowski RJ, Edwards SJ, Bygren P, Heinegård D, Hudson BG (1984) Goodpasture antigen of the glomerular basement membrane: Localization to noncollagenous regions of type IV collagen. Proc Natl Acad Sci USA 81:3838–3842
Wieslander J, Langeveld J, Butkowski R, Jodlowski M, Noelken M, Hudson BG (1985) Physical and immunochemical studies of the globular domain of type IV collagen. Cryptic properties of the Goodpasture antigen. J Biol Chem 260:8564–8570
Wilson CB, Dixon FJ (1973) Anti-glomerular basement membrane antibody-induced glomerulonephritis. Kidney Int 3:74–89
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sado, Y., Kagawa, M., Naito, I. et al. Properties of bovine nephritogenic antigen that induces anti-GBM nephritis in rats and its similarity to the Goodpasture antigen. Virchows Archiv B Cell Pathol 60, 345–351 (1991). https://doi.org/10.1007/BF02899566
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02899566