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Establishment and characterization of an immature epithelial cell line (ENU-T-1) derived from a rat nephroblastoma

Virchows Archiv B volume 57, Article number: 383 (1989) Cite this article

Summary

A new cell line designated ENU-T-1 has been established from a xenotransplanted experimental rat nephroblastoma. The cultured cells are spindle-shaped or polygonal and are arranged in a wavy fashion morphologically similar to cultured embryonal renal epithelial cells. The cells exhibit a number of epithelial characteristics. Enzyme histochemistry gives positive reactions for gammaglutamyltranspeptidase and alkaline phosphatase, both of which are present in renal tubular epithelial cells. Immunofluorescence studies show positive reactions for vimentin and cytokeratin. When inoculated into athymic nude mice, the cultured cells form tumors composed of sheets of epithelial cells with focal tubular formation.

This cell line may be of value in studying differentiation of nephroblastoma, and possibly normal nephrogenesis.

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References

  • Altmannsberger M, Osborn M, Schaefer H, Schauer A, Weber K (1984) Distinction of nephroblastomas from other childhood tumors using antibodies to intermediate filaments. Virchows Arch [Cell Pathol] 45:113–124

    CAS  Google Scholar 

  • Babcock VI, Southam CM (1967) Stable cell line of rat nephroblastoma in tissue culture. Soc Exp Biol Med 124:217–219

    CAS  Google Scholar 

  • Caspersson T, Zech L, Johansson C, Modest EJ (1970) Identification of human chromosomes by DNA-binding fluorescent agents. Chromosoma 30:215–227

    PubMed  Article  CAS  Google Scholar 

  • Cereijido M, Ehrenfeld J, Fernendez-Castelo S, Meza I (1981) Fluxes, junctions, and blisters in cultured monolayers of epithelioid cells (MCDK). Ann NY Acad Sci 372:422–441

    PubMed  Article  CAS  Google Scholar 

  • Chang CC, Trosko JE, El-Fouly MH, Gibson-D’Ambrosio RE, D’Ambrosio SM (1987) Contact insensitivity of a subpopulation of normal human fetal kidney epithelial cells and of human carcinoma cell lines. Cancer Res 47:1634–1645

    PubMed  CAS  Google Scholar 

  • Chen TR (1977) In situ detection of mycoplasm contamination in cell cultures by fluorescent Hoechst 33258 stain. Exp Cell Res 104:255–262

    PubMed  Article  CAS  Google Scholar 

  • Committee for a standardized karyotype of Rattus norvegicns (1973) Standard karyotype of the Norway rat, Rattus norvegiens. Cytogenet Cell Genet 12:199–205

    Article  Google Scholar 

  • Douglas EC, Williams JA, Green AA, Look T (1985) Abnormalities of chromosomes 1 and 11 in Wilms’ tumor. Cancer Genet Cytogenet 14:331–338

    Article  Google Scholar 

  • Fearon ER, Vogelstein B, Feinberg AP (1985) Somatic deletion and duplication of genes on chromosome 11 in Wilms’ tumours. Nature 309:176–178

    Article  Google Scholar 

  • Fuchs E, Green H (1980) Changes in keratin gene expression during terminal differentiation of the keratinocyte. Cell 19:1033–1042

    PubMed  Article  CAS  Google Scholar 

  • Gibson-D’Ambrosio RE, Samuel M, Chang CC, Trosko JE, D’Ambrosio SM (1987) Chracteristics of long-term human epithelial cell cultures derived from normal fetal kidney. In Vitro 23:279–287

    CAS  Google Scholar 

  • Gilbert SF and Migeon BR (1975) D-valine as a selective agent for normal human and rodent epithelial cells in culture. Cell 5:11–17

    PubMed  Article  CAS  Google Scholar 

  • Grobstein C (1955) Trans-filter induction of tubules in mouse metanephric mesenchyme. Exp Cell Res 10:424–440

    Article  Google Scholar 

  • Hard GC (1984) Tumor biology; In vitro culture and transplantation models of Wilms’ tumor. In: Prochedly C, Baun ES (eds) Wilms’ tumor, Clinical and biological manifestations. Elsevier Co, New York, pp 191–241

    Google Scholar 

  • Hard GC, Brown C, Wrobleski JB, Noble RL (1982) In vitro isolation and establishment of new embryonal lines from rat nephroblastoma. In Vitro 18:872–883

    PubMed  CAS  Article  Google Scholar 

  • Holthoefer H, Miettinen A, Lehto V-P, Lehtonen E, Virtanen I (1984) Expression of vimentin and cytokeratin types of intermediate proteins in developing and adult human kidneys. Lab Invest 50:552–559

    Google Scholar 

  • Holthoefer H, Miettinen A, Paasivuo R, Lehto V-P, Linder E, Alfthan O, Virtanen I (1983) Cellular origin of renal cell carcinomas: a fluorescence microscopic study of kidneyspecific antibodies, antiintermediate filament antibodies, and lectins. Lab Invest 49:317–326

    Google Scholar 

  • Kumar S, Harrison CJ, Heighway J, Marsden HB, West DC, Jones PM (1987) A cell line from Wilms’ tumor with deletion in short arm of chromosome 11. Int J Cancer 40:499–504

    PubMed  Article  CAS  Google Scholar 

  • Kuroki T (1973) Colony formation of mammalian cells on agar plates and its application to Lederberg’s replica. Exp Cell Res 80:55–62

    PubMed  Article  CAS  Google Scholar 

  • Lane EB (1982) Monoclonal antibodies provided specific intramolecular markers for the study of epithelial tonofilament organization. J Cell Biol 92:665–673

    PubMed  Article  CAS  Google Scholar 

  • Lane EB, Klymkowsky MW (1981) Epithelial tonofilaments: Investigating their form and function using monoclonal antibodies. Cold Spring Harbor Symp Quart Biol 46:387–402

    Google Scholar 

  • Mierau GW, Beckwith JB, Weeks DA (1987) Ultrastructure and histogenesis of the renal tumors of childhood: An overview. Ultrastruct Pathol 11:313–333

    PubMed  Article  CAS  Google Scholar 

  • Moll R, Franke WW, Schiller DL (1982) The catalog of human cytokeratins; Patterns of expression in normal epithelia, tumor, and cultured cells. Cell 31:11–24

    PubMed  Article  CAS  Google Scholar 

  • Ohaki Y (1989) Renal tumors induced transplacentally in the rat by N-ethylnitrosourea. Pediatr Pathol 9:19–33

    PubMed  Article  CAS  Google Scholar 

  • Oshimura M, Koi M (1987) The study of tumor suppression III, transfer of human chromosome 11 to Wilms’ tumor and rhabdomyosarcoma cell lines. Proc Jap Cancer Association 46th annual meeting [Abstr, in Japanese] 456

  • Pirthle EC, Mengeling WL, Cheville NF (1970) Initiation and characterization of two porcine embryonal nephroma cell lines. Am J Vet Res 31:1601–1608

    Google Scholar 

  • Pitz S, Moll R, Stoerkel S, Thoenes W (1987) Expression of intermediate filament proteins in subtypes of renal cell carcinomas and renal oncocytomas. Distinction of two classes of renal cell tumors. Lab Invest 56:642–653

    PubMed  CAS  Google Scholar 

  • Riccardi VM, Hittner HM, Francke U, Yunis JJ, Ledbetter D, Borges W (1980) The aniridia-Wilms’ tumor association; The critical role of chromosome band 11p13. Cancer Genet Cytogenet 2:131–137

    Article  Google Scholar 

  • Slater RM, de Kraker J (1982) Chromosome number 11 and Wilms’ tumor. Cancer Genet Cytogenet 5:237–245

    PubMed  Article  CAS  Google Scholar 

  • Taub M, Livingstone D (1981) The development of serum-free hormone-supplemented media for primary kidney cultures and their use in examining renal function. Ann NY Acad Sci 81:406–421

    Article  Google Scholar 

  • Van Muijen GNP, Ruiter DJ, Warnaar SO (1987) Coexpression of intermediate filament polypeptides in human fetal and adult tissues. Lab Invest 57:359–369

    PubMed  Google Scholar 

  • Weissman BE, Saxon PJ, Pasquale SR, Jones GR, Geiser AG, Stanbridge EJ (1987) Introduction of a normal human chromosome into Wilms’ tumor cell line controls its tumorigenic expression. Science 236:175–180

    PubMed  Article  CAS  Google Scholar 

  • Williams RD (1980) Human urologie cancer cell lines. Invest Urol 19:357–363

    CAS  Google Scholar 

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Affiliations

  1. Department of Pathology, Yokohama City University, School of Medicine, Fukuura 3-9, Kanazawa-ku, 236, Yokohama, Japan

    Yoji Nagashima, Yoshiharu Ohaki & Kazuaki Misugi

  2. Department of Cell Biology, Kihara Institute for Biological Research, Yokohama City University, Yokohama, Japan

    Makoto Umeda

  3. Laboratory of Cytogenetics, Kanagawa Cancer Center Research Institute, Yokohama, Japan

    Mitsuo Oshimura

Authors
  1. Yoji Nagashima
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  2. Yoshiharu Ohaki
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  3. Makoto Umeda
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  4. Mitsuo Oshimura
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  5. Kazuaki Misugi
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Nagashima, Y., Ohaki, Y., Umeda, M. et al. Establishment and characterization of an immature epithelial cell line (ENU-T-1) derived from a rat nephroblastoma. Virchows Archiv B Cell Pathol 57, 383 (1989). https://doi.org/10.1007/BF02899105

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  • DOI: https://doi.org/10.1007/BF02899105

Key words

  • Nephroblastoma
  • Nephroblasts
  • Cell line