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Effects of methanolic extracts from broad beans on cellular growth and antioxidant enzyme activity

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Environmental Health and Preventive Medicine Aims and scope

Abstract

Objective

There are several reports of cellular-aging-dependent alterations in the antioxidant capacity of human fibroblasts. Fibroblasts show slower the growth rate at late passages (referred to hereafter as old cells) than at early passages (referred to hereafter as young cells). Antioxidants may control cellular growth by modulating reactive oxygen species (ROS). Methanolic extracts from broad beans (MEBB) contain phenolic compounds and have ROS-scavenging activities. In this study, we investigated the effects of MEBB on cellular growth and antioxidant levels in normal human lung fibroblasts.

Methods

To determine cytosolic superoxide dismutase (SOD) activities, cytosolic glutathione peroxidase (GSH-Px) activities, catalase activities, reduced glutathione (GSH) concentrations, and growth rate, MEBB treatments were performed on young and old cells.

Results

In young and old cells treated with 120 μg/ml MEBB, the growth rates increased by 28.1 and 15.2%, respectively, compared with controls. The MEBB treatment of young cells caused a 62.5% increase in SOD activity, but the treatment of old cells caused a 39.5% decrease. The catalase activities of the young and old cells treated with MEBB were equal to those of control cells. Young and old cells treated with MEBB were equal to the control cells in terms of GSH-Px activity. The GSH concentrations in the young and old cells treated with 120 μg/ml MEBB increased by 22.1 and 45.9%, respectively.

Conclusion

These studies elucidated a new cellular growth mechanism whereby human lung fibroblasts modulate intracellular GSH levels via the action of MEBB.

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Authors and Affiliations

  1. Nutrition Section, Yms Laboratory, Gifu, Japan

    Mizue Okada

  2. Laboratory on Ageing and Health Management, Aichi Prefectural College of Nursing and Health, Tohgoku, Kamishidami, Moriyama, 463-8502, Nagoya, Japan

    Yoshinori Okada

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  1. Mizue Okada
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Correspondence to Mizue Okada.

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Okada, M., Okada, Y. Effects of methanolic extracts from broad beans on cellular growth and antioxidant enzyme activity. Environ Health Prev Med 12, 251–257 (2007). https://doi.org/10.1007/BF02898032

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  • DOI: https://doi.org/10.1007/BF02898032

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