Abstract
Resistance of transformed and non-transformed spring oilseed rape cv. HM-81 to the infection with cauliflower mosaic virus (CaMV), turnip yellow mosaic virus (TYMV) and turnip mosaic virus (TuMV) was studied, to determine the influence of transformation on susceptibility of plants to viruses. For experiments the non-segregating R 1 generation of primary transformant HM-81-JZ and control plants of cv. HM-81 were used. The primary transformant was obtained by inoculation of stems withAgrobacterium rhizogenes 15834. All transformed plants of R 1 generation had typically „transformed“ phenotype. No significant differences were revealed in the resistance of both transformed and non-transformed plants to each virus, as proved by qualitative and quantitative ELISA and visual evaluation of symptoms. Transformed plants infected with turnip yellow mosaic virus showed significantly lower reduction of green mass yield than non-transformed. In the case of CaMV and TuMV infection reduction of yield of transformed and non-transformed plants was almost the same.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Antoniw, F. J., Ooms, G., White, R. F., Wullems, G. J., V. Vlotendoting, L.: Pathogenesis-related proteins in plants and tissues ofNicotiana tabacum transformed byAgrobacterium tumefaciens. - Plant mol. Biol.2: 317–320,1983.
Dusbábková, J., Nečásek, J., Hrouda, M.: [Transformation of oilseed rape with T-DNA ofAgrobac- terium plasmids.] (In Czech). - Genet. Šlecht. (Praha)25:1–9, 1989.
Gasser, R., Kern, H., Défago, G.: Resistance of tobacco toThielaviopsis basicola under tissue culture conditions and increased susceptibility after transformation withAgrobacterium tumefaciens T-DNA. - J. Phytopathol.123: 105–114, 1988.
Hrouda, M., Dusbábková, J., Nečásek, J.: Detection of Ri T-DNA in transformed oilseed rape regenerated from hairy roots. - Biol. Plant. 30: 234–236, 1988.
Linthorst, H. J. M., Meuwissen, R. L. J., Kaufmann, S., Bol, J. F.: Constitutive expression of pathogenesis-related proteins PR-1, GRP and PR-S in tobacco has no effect on virus infection. - Plant Cell1: 285–291, 1989.
Mugnier, J.: Exemples d'étude d'organismes parasites des racines transformées parAgrobacterium rhizogenes. -Cryptogamie Mycol.9: 233–238, 1988.
Ooms, G., White, R. F., Antoniw, J. F., Gibson, R. W.:Agrobacterium rhizogenes - transformed and untransformed tobacco plants have similar pathogenesis related protein concentrations and similar suceptibility to virus infection. - Plant Sci. Lett.35.: 169–173, 1984.
Polák, J., Křístek, J.: Use of horseradish-peroxidase labelled antibodies in ELISA for plant virus detection. - J. Phytopathol.122.: 200–207, 1988.
Polák, J., Špak, J.: The incidence of two serologically distinct strains of turnip yellow mosaic virus on cultivated cruciferous plants in Czechoslovakia. - Arch. Phytopathol. Pflanzenschutz23: 269–274, 1987.
Polák, Z., Špak, J., Procházková, Z., Venclová, D.: [Yield losses of winter rape and turnip rape after infection with some plant viruses.] (in Czech). - Sborník ÚVTIZ- Ochrana Rostlin25: 1–7, 1989.
Procházková, Z., Polák, Z.: [Comparison of the infectivity of the two isolates of turnip mosaic virus.] (inCzech). - Sborník ÚVTIZ - Ochrana Rostlin21: 251–254, 1985.
Schmülling, T., Schell, J., Spena, A.: Single genes fromAgrobacterium rhizogenes influence plant development. - EMBO J.7: 2621–2629, 1988.
Špak, J., Procházková, Z., Polák, Z.: [Resistance of spring and winter rape cultivars with different glucosinolate contents to the turnip mosaic virus.](In Czech). - Sborník ÚVTIZ - Ochrana Rostlin23: 87–93, 1987.
Špak, J.: [Purification and serodiagnosis of cauliflower mosaic virus],(In Czech).] - Sbornik ÚVTIZ - Ochrana Rostlin25: 241–247, 1989.
Tepfer, D.: Transformation of several species of higher plants byAgrobacterium rhizogenes: Sexual transmission of the transformed genotype and fenotype. - Cell37: 959–967,1984.
Vilaine, F., Casse-Delbart, F.: Independent induction of transformed roots by the TL and TR regions of the Ri plasmid of agropine type Agrobacteriumrhizogenes. - Mol. gen. Genet.206:17–23, 1987.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Špak, J., Dusbábková, J., Kubelková, D. et al. Resistance of transformed and non-transformed oilseed rape cv. HM-81 to the infection with cauliflower mosaic, turnip yellow mosaic and turnip mosaic viruses. Biol Plant 33, 234–239 (1991). https://doi.org/10.1007/BF02897889
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02897889