Abstract
Morphogenic callus cultures were obtained from 7–10 days old immature embryo explants on Murashige and Skoog and Gamborg’s medium supplemented with 2,4-D. In the initial stages of culture the frequency of shoot formation varied from 28% to 65%. After 5 to 6 months of subculturing, the frequency of shoot formation was reduced to 14%. In the initial stages of culture, growth hormones do not seem to be very important for regeneration. Cultures from young and old non-differentiating calli, and calli with shoot and/or root formation at different intervals were analysed for isozymes of esterase, peroxidase and acid phosphatase for studying the morphogenic capacity. With the development of shoot/root, changes in isozymes takes place but no specific isozyme(s) could be related to the process of induction of morphogenesis.
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Chawla, H.S. Regeneration potentiality and isozymic variations during morphogenesis of barley callus. Biol Plant 33, 175–180 (1991). https://doi.org/10.1007/BF02897878
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DOI: https://doi.org/10.1007/BF02897878