Skip to main content
SpringerLink
Log in

Nitrogen metabolism inErica and soybean, two species differing by their sensitivity to inorganic N source

  • Published:
Biologia Plantarum

Abstract

Growth of soybean was not altered, whatever the inorganic N-source (NO3, NH4 or a NO3/NH4 mixture); conversely, growth of Erica x darleyensis plants in vitro decreased more in. NH4 medium than in a NO3 medium, compared to a NO3/NH4 medium. The GS/GOGAT pathway (in NH4 medium), the nitrate and nitrite reductase activities (in NO3 medium), as the contents in free nitrogenous forms and total nitrogen (in NO3 and NH4 media) were not more altered in Erica than in soybean, compared to a NO3/NH4 medium. PEPCase activity was the highest in soybean irrespective of the N-treatments; the involvement of PEPCase in N-metabolism could be explained by its function in ionic and osmotic balances rather than its function in supplying carboxylates as acceptors for NH4-assimilation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Arnozis, P. A., Findenegg, G. R.: Electrical charge balance in the xylem sap of beet and sorghum plants were grown with either NO3 or NH4 nitrogen. -J. Plant Physiol.125:441–449, 1986.

    CAS  Google Scholar 

  • Arnozis, P. A., Nelemans, J. A., Findenegg, G. R.: Phosphoenolpyruvate carboxylase activity in plants grown with either NO3 or NH4 as inorganic N source. -J. Plant Physiol. 132:23–27, 1988.

    CAS  Google Scholar 

  • Bergmeyer, H. U.: Methods of enzymatic analysis. Vol. 2. Pp. 613–617. Acad. Press, New York 1974.

    Google Scholar 

  • Blackwood, C. Miflin, B. J.: The effect of nitrate and ammonium feeding on carbon assimilation in maize. -J. exp. Bot.27:735–747, 1976.

    Article  CAS  Google Scholar 

  • Boucaud. J., Billard, J. P.: La glutamine synthetase duSuaeda maritima: Actionin vivo etin vitro du NaCl.-Physiol. Plant.53:558–564, 1981.

    Article  CAS  Google Scholar 

  • Bradford, M. M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. -Anal. Biochem.72:248–253, 1976.

    Article  PubMed  CAS  Google Scholar 

  • Cataldo, D. A., Hardon. M.. Schrader, L. E., Youngs, V. L.: Rapid colorimetric determination of NO3 in plant tissue by nitration of salicylic acid. -Commun. Soil Sci. Plant Anal.6:71–80, 1975.

    CAS  Google Scholar 

  • Cox, W. J., Reisenauer, H. M.: Growth and ion uptake by wheat supplied nitrogen as nitrate, ammonium or both. -Plant Soil38:363–380, 1973.

    Article  CAS  Google Scholar 

  • Davies, D. D.: The essential role of phosphoenolpyruvate carboxylase in plant metabolism.-Annu. Rev. Plant Physiol.30:131–158, 1979.

    Article  CAS  Google Scholar 

  • Fentem, P. A., Lea, P. J., Stewart, G. R.: Ammonia assimilation in roots of nitrate and ammonia growthHordeum vulgare. -Plant Physiol.71:496–501, 1983.

    PubMed  CAS  Google Scholar 

  • Guerrier, G.: Activité de quelques enzymes du cycle de l'azote lors de la germination en milieu salé.-Fyton48:85–91, 1988a.

    CAS  Google Scholar 

  • Guerrier, G.: Capacités Phosphoénolpyruvate carboxylase et Malatc déshydrogénase extraites des plantules germées en milieu salé: des paramètres de l'écophysiologie de la plante? -Seed Sci.-Technol.16:571–578, 1988b.

    CAS  Google Scholar 

  • Guerrier, G., Beaujard, F., Viémont, J. D.: Influence de la concentration de la solution nutritive dans la phase de réinsertionin vivo des vitroplants d'Erica x darleyensis. 2) Relations entre les fractions libres azotées et les capacités nitrate réductate et glutamate déshydrogénase. -Plant Soil84:337–345, 1985.

    Article  CAS  Google Scholar 

  • Kirkby, E. A., Knight, A.: Influence of the level of nitrate nutrition on ion uptake and assimilation, organic acid accumulation and cation-anion balance in whole tomato plants. -Plant Physiol.60:349–353, 1977.

    PubMed  CAS  Google Scholar 

  • Magalhaes, J.-R., Wilcox, G. E.: Ammonium toxicity development in tomato plants, relative to nitrogen form and light intensity. -J. Plant Nutr.7:1447–1496, 1974.

    Google Scholar 

  • Oaks, A., Hirel, B.: Nitrogen metabolism in roots. -Annu. Rev. Plant Physiol.36:345–365, 1985.

    Article  CAS  Google Scholar 

  • Raven, J. A., Smith, F. A.: Nitrogen assimilation and transport in vascular land plants in relation to intracellular pH regulation. -New Phytol.75:415–431, 1976.

    Article  Google Scholar 

  • Robin, P.: Etude de quelques conditions d'extraction de la nitrate réductase des racines et des feuilles de plantules de mais.-Physiol. vég.17:45–54, 1979.

    CAS  Google Scholar 

  • Salsac, L., Chailou, S., Morot-Gaudry, J. F., Lesaint, C., Jolivet, E.: Nitrate and ammonium nutrition in plants. -Plant Physiol. Biochem.25:805–812, 1987.

    Google Scholar 

  • Schweizer, P., Erismann, K. H.: Effect of nitrate and ammonium nutrition of non nodulatedPhaseolus vulgaris on PEPCase and pyruvate kinase activity. -Plant Physiol.78:455–458, 1985.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. FRD Biotechnologies, Ensemble Scientifique, 2 Bd Lavoisier, 490 45, Angers Cedex, France

    G. Guerrier

Authors
  1. G. Guerrier
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Guerrier, G. Nitrogen metabolism inErica and soybean, two species differing by their sensitivity to inorganic N source. Biol Plant 33, 468–474 (1991). https://doi.org/10.1007/BF02897722

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02897722

Additional index words

Search

Navigation